Navegando por Autor "Silva, Rosangela Maria Ferreira da Costa e"

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Bioactive glass containing 90% SiO2 in hard tissue engineering : an in vitro and in vivo characterization study.
(2019) Lehman, Luiz Felipe Cardoso; Noronha, Mariana Saturnino de; Diniz, Ivana Márcia Alves; Silva, Rosangela Maria Ferreira da Costa e; Andrade, Ângela Leão; Lima, Luiz Fernando de Sousa; Alcântara, Carlos Eduardo Pinto de; Domingues, Rosana Zacarias; Ferreira, Anderson José; Silva, Tarcília Aparecida da; Mesquita, Ricardo Alves de
Bioactive glass has been proved to have many applications in bioengineering due to its bone regenerative properties. In this work, an innovative, highly resorbable bioactive glass containing 90% SiO2 (BG90) to be used as a bone substitute was developed. The BG90 was synthetized by the sol–gel process with the dry step at room temperature. The biomaterial showed in vitro and in vivo bioactivities even with silica content up to 90%. Moreover, the BG90 presented high porosity and surface area due to its homogenously interconnected porous network. In vitro, it was observed to have high cell viability and marked osteoblastic differentiation of rat bone marrow‐derived cells when in contact with BG90 ion extracts. The BG90 transplantation into rat tibia defects was analysed at 1, 2, 3, 4, 7, and 10 weeks post‐operatively and compared with the defects of negative (no graft) and positive (autogenous bone graft) controls. After 4 weeks of grafting, the BG90 was totally resorbed and induced higher bone formation than did the positive control. Bone morphogenetic protein 2 (BMP‐2) expression at the grafting site peaked at 1 week and decreased similarly after 7 weeks for all groups. Only the BG90 group was still exhibiting BMP‐2 expression in the last experimental time. Our data demonstrated that the BG90 could be an attractive candidate to provide useful approaches in hard‐tissue bioengineering.
Contribuição química ao estudo de compostos usados no tratamento da hipersensibilidade dentinária.
(2022) Ribeiro, Renata Nardy; Andrade, Ângela Leão; Silva, Rosangela Maria Ferreira da Costa e; Vasconcellos, Walison Arthuso
Hipersensibilidade dentinária (HD) é uma condição clínica dolorosa comum, geralmente associada às superfícies dentinárias expostas, que fazem com que seu sistema tubular fique aberto. Isso ocasiona um movimento do fluido e ativa uma resposta neural na polpa. Embora ela seja uma das queixas mais comuns de pacientes, não existem diretrizes universalmente aceitas para o diagnóstico diferencial, nem modalidades de tratamento confiáveis. Além disso, os mecanismos pelos quais os diferentes materiais atuam ainda não são muito claros e esse conhecimento é primordial para um tratamento seguro e efetivo. O objetivo dessa revisão de literatura foi, por meio de uma busca científica em bases de dados (PubMed e Google Scholar) e de um conhecimento de química básica, explicar alguns mecanismos químicos envolvidos na mineralização e desmineralização da dentina e a ação de diferentes compostos usados no tratamento da HD, subsidiando os profissionais a encontrar o melhor tratamento para cada paciente.
Desenvolvimento, caracterização e atividade antimicrobiana de creme dental contendo vidro bioativo e extratos vegetais para controle da hipersensibilidade dentinária.
(2022) Ribeiro, Renata Nardy; Andrade, Ângela Leão; Silva, Rosangela Maria Ferreira da Costa e; Vasconcellos, Walison Arthuso; Andrade, Ângela Leão; Martins, Darliane Aparecida; Santos, Vagner Rodrigues
Hipersensibilidade dentinária (HD) é uma condição clínica comum, geralmente associada a superfícies dentinárias expostas. Embora ela seja uma das queixas mais comuns de pacientes em clínicas odontológicas, não existem diretrizes universalmente aceitas para o diagnóstico diferencial, nem modalidades de tratamento confiáveis para essa condição. Além disso, os mecanismos pelos quais os diferentes materiais atuam ainda não são muito claros e esse conhecimento é primordial para um tratamento seguro e efetivo. O objetivo desse trabalho foi desenvolver uma formulação de creme dental contendo extrato de ginseng brasileiro, extrato de erva doce e vidro bioativo do compósito de Equisetum hyemalle. Esse creme dental foi preparado para ser usado na redução da sensibilidade dentinária. Experimentos iniciais de estabilidade acelerada realizados a temperatura ambiente e a 50°C (estabilidade acelerada) comprovaram a estabilidade físico-química das formulações desenvolvidas. Os extratos de ginseng e erva doce, quando adicionados ao creme dental base, apresentaram atividade antimicrobiana contra o microrganismo S. mutans. Imagens de microscopia eletrônica de varredura, evidenciou que o creme dental com vidro bioativo foi capaz de fechar os túbulos dentinários, sugerindo eficácia no tratamento da HD.
Enhanced mechanical properties, anti-biofilm activity, and cytocompatibility of a methacrylate-based polymer loaded with native multiwalled carbon nanotubes.
(2022) Rodrigues, Ricardo Antonio Alpino; Silva, Rosangela Maria Ferreira da Costa e; Ferreira, Luiza de Almeida Queiroz; Branco, Natália Tavares Teixeira; Ávila, Érick de Souza; Peres, Anderson Maia; Braga, Weslley Fernandes; Dias, Augusto César Sette; Andrade, Ângela Leão; Dibb, Regina Guenka Palma; Magalhães, Cláudia Silami de; Ladeira, Luiz Orlando; Silveira, Rodrigo Richard da; Moreira, Allyson Nogueira; Martins Júnior, Paulo Antônio; Yamauti, Mônica; Diniz, Ivana Márcia Alves
Objectives: We aimed to optimize the mechanical and biological properties of a conventional methacrylate-based dental polymer by loading it with double- and triple-walled carbon nanotubes as growth (DTWCNTG). Methods: A formulation of bisphenol A-glycidyl methacrylate and triethylene glycol dimethacrylate (mass ratio = 2:1) was mixed with DTWCNTG at concentrations of 0.0% (control), 0.001%, 0.005%, and 0.010%. The concentrations were physicochemical and morphologically evaluated, and antibacterial activity was assessed by seeding a Streptococcus mutans strain (ATCC 25175) on the experimental polymeric surfaces. Cellular survival and osteodifferentiation were evaluated in epithelial (HaCat) and preosteoblast cells (MC3T3-E1). Results: The 0.001% DTWCNTG concentration yielded higher compressive strength, elastic modulus, flexural strength, flexural modulus, water sorption, and solubility than the control. The degree of conversion and color did not significantly change with a low amount of DTWCNTG incorporated into the polymer. Antibacterial activity significantly improved when tested on the 0.001% DTWCNTG discs. No groups showed cytotoxicity in a short-term analysis and adding DTWCNTG favored MC3T3-E1 mineralization over the control, particularly in the 0.001% formulation. Significance: The micro-addition of 0.001% DTWCNTG confers mechanical resistance, antimicrobial properties, and bioactivity to methacrylate-based polymers without significantly compromising color. Incorporating DTWCNTG improved dental composite properties and could be a biomodified material for minimally invasive procedures.
Equisetum hyemale‑derived unprecedented bioactive composite for hard and soft tissues engineering.
(2022) Silva, Rosangela Maria Ferreira da Costa e; Diniz, Ivana Márcia Alves; Gomes, Natália Aparecida; Silva, Guilherme Jorge Brigolini; Ferreira, José Maria da Fonte; Freitas Filho, Rubens Lucas de; Freitas, Erico Tadeu Fraga; Martins, Darliane Aparecida; Domingues, Rosana Zacarias; Andrade, Ângela Leão
Although Bioactive Glasses (BGs) have been progressively optimized, their preparation often still involves the use of toxic reagents and high calcination temperatures to remove organic solvents. In the present work, these synthesis related drawbacks were overcome by treating the ashes from the Equisetum hyemale plant in an ethanol/water solution to develop a bioactive composite [glass/ carbon (BG-Carb)]. The BG-Carb was characterized by scanning electron microscopy, and transmission electron microscopy; and its chemical composition was assessed by inductively coupled plasmaoptical emission spectroscopy. Brunauer–Emmett–Teller gas adsorption analysis showed a specifc surface area of 121 m2 g−1. The formation of hydroxyapatite (HA) surface layer in vitro was confrmed by Fourier-transform infrared spectroscopy analysis before and after immersion in simulated body fuid (SBF) solution. The Rietveld refnement of the XRD patterns and selected area electron difraction analyses confrmed HA in the sample even before immersing it in SBF solution. However, stronger evidences of the presence of HA were observed after immersion in SBF solution due to the surface mineralization. The BG-Carb samples showed no cytotoxicity on MC3T3-E1 cells and osteodiferentiation capacity similar to the positive control. Altogether, the BG-Carb material data reveals a promising plant waste-based candidate for hard and soft tissue engineering.
Glass-ionomer-propolis composites for caries inhibition: flavonoids release, physical-chemical, antibacterial and mechanical properties.
(2019) Andrade, Ângela Leão; Lima, Adriana M.; Santos, Vagner Rodrigues; Silva, Rosangela Maria Ferreira da Costa e; Barboza, Ana Paula Moreira; Neves, Bernardo Ruegger Almeida; Vasconcellos, Walison Arthuso; Domingues, Rosana Zacarias
The addition of propolis extract (PE) to the glass ionomer results in an adhesive material for restorative treatment, with interesting properties mainly due to the flavonoids contained in the propolis extract. However, no study of the flavonoid release profile in these materials was reported. This work studies the flavonoid release profile in such materials aiming to contribute to the future synthesis of optimized devices adept to prolong the efficacy of the drug. The study involved the synthesis and study of the physicochemical, antibacterial and mechanical properties of glass ionomer cement (GIC) and glassionomer- propolis composites (GIC-PE). The samples were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analyses. The released concentration of flavonoids, the antimicrobial activity and the compressive strength were also evaluated. Antimicrobial activity was assessed against Streptococcus mutans, Streptococcus salivarius, and Candida albicans, common pathogens in the mouth. The results indicate that the antibacterial activity of GIC-PE samples is closely correlated with the release of flavonoids. The method used to prepare the composite GIC-PE leads to an initial drug delivery burst effect able to diminish partially the population of bacteria tested. The mechanical properties and thermal stability of GIC-PE are higher than those of the GIC and are clearly related to its microstructure. This study is clinically significant because the addition of propolis extract (PE) to the GIC resulted in a novel differentiated product with enhanced mechanical and antimicrobial properties compared to the GIC.
Preparation of magnetoliposomes with a green, low-cost, fast and scalable methodology and activity study against S. aureus and C. freundii bacterial strains.
(2018) Silva, Rosangela Maria Ferreira da Costa e; Lara, Luciano Roni Silva; López Aguilar, Jorge Luis; Andrade, Ângela Leão; Oliveira, Junnia A. C.; Takahashi, Jacqueline Aparecida; Vieira, Henriete da Silva; Matencio, Tulio; Stumpf, Humberto Osório; Domingues, Rosana Zacarias
A novel, fast, low-cost and scalable methodology to prepare stable magnetoliposomes (MGLs), without the use of organic solvents, is described. The concept of the work is based on the dual use of soy lecithin associated to a new liposome preparation methodology. Soy lecithin was used to coat the nanoparticles of magnetite (Fe3 O4 @lecithin) and for encapsulation of Fe3 O4 @lecithin (Lip-Fe3 O4 @lecithin). Liposomes with size less than 160 nm, polydispersity index of 0.25 and zeta potential of -41 mV, were prepared with the use of autoclave and sonication. The liposomal formulations containing magnetite and stigmasterol (Lip-Fe3 O4 @lecithin, Lip-Stigma and Lip-Stigma-Fe3 O4 @lecithin) were shown to be promising for the application as antibacterial. The liposomal formulation and magnetite were characterized by the following techniques: conventional and high-resolution transmission electron microscopy (TEM/HRTEM), energy-filtered transmission electron microscopy (EFTEM), proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRPD), dynamic light scattering (DLS) and zeta potential. The Lip-Fe3 O4 @lecithin had a minimum inhibitory concentration (MIC) of 8.4 µg mL-1 in the presence of 200 Oe magnetic field against S. aureus.
Síntese, caracterização físico-química e avaliação da hematocompatibilidade, in vitro, de nanopartículas de magnetita.
(2022) Gomes, Aline Alexandrina; Andrade, Ângela Leão; Valverde, Thalita Marcolan; Domingues, Rosana Zacarias; Andrade, Ângela Leão; Rocha, Diego Gomes; Silva, Rosangela Maria Ferreira da Costa e
Pesquisas em nanobiotecnologia têm evoluído nos últimos anos. Na área médica, por exemplo, nanopartículas magnéticas têm sido estudadas como agentes de contraste, bioensaios magnéticos, e como carreadoras de fármacos associados à terapia de hipertermia. Sínteses de nanopartículas magnéticas estão sendo feitas com objetivo de obter materiais superparamagnéticos, com formas uniformes, no caso esféricas, potencial magnético e de aquecimento satisfatórios sob campo magnético oscilante, externamente aplicado, e capacidade de adsorver fármacos na sua estrutura para serem usadas como material de transporte para células tumorais. Essas caraterísticas fazem com que essas nanopartículas magnéticas possam ser classificadas como materiais multimodais. Estudos de hipertermia com nanopartículas magnéticas podem ser usados de forma auxiliar aos tratamentos convencionais (quimioterapia e radioterapia). A finalidade do uso da hipertermia, é diminuir os efeitos colaterais dessas terapias convencionais, já que, a hipertermia consiste em um aumento da temperatura local que pode enfraquecer ou matar as células doentes além de promover a liberação de fármacos diretamente para áreas doentes, potencializando o tratamento. A sensibilidade de células tumorais frente ao tratamento por hipertermia pode ser explicada pelo fato que o tecido tumoral apresenta alterações importantes no microambiente tecidual que prejudica a dissipação do calor. Para que um material possa ser administrado em um organismo vivo, como os materiais usados em hipertermia, é importante avaliar previamente a sua biocompatibilidade por meio de ensaios de biologia celular. Sendo assim, o objetivo desse trabalho foi sintetizar, funcionalizar e revestir nanopartículas magnéticas com polímeros orgânicos a fim de estabilizá-las em soluções aquosas antes do seu uso. Uma análise multivariada foi feita para a obtenção das condições que produzem os menores tamanhos de nanopartículas. A validação do modelo matemático, obtido nessa análise, foi feito pela análise de variância (ANOVA). Caracterizações físico – químicas das partículas sintetizadas e revestidas foram feitas por TEM; HRTEM; Hipertermia; FTIR; DLS; PdI e Potencial Zeta. Adicionalmente, foi realizada uma avaliação biológica por meio do ensaio de hematocompatibilidade in vitro.
Theoretical and experimental studies of the controlled release of tetracycline incorporated into bioactive glasses.
(2018) Andrade, Ângela Leão; Militani, Isabela Aparecida; Almeida, Katia Júlia de; Belchior, Jadson Cláudio; Reis, Sérgio C. dos; Silva, Rosangela Maria Ferreira da Costa e; Domingues, Rosana Zacarias
Several authors have studied the release profile of drugs incorporated in different devices. However, to the best of our knowledge, although many studies have been done on the release of tetracycline, in these release devices, no study has investigated if the released compound is actually the tetracycline, or, instead, a degraded product. This approach is exploited here. In this work, we analyse the influence of two drying methods on the tetracycline delivery behaviour of synthesised glasses using the sol-gel process. We compare the drying methods results using both theoretical models and practical essays, and analyse the chemical characteristic of the released product in order to verify if it remains tetracycline. Samples were freeze-dried or dried in an oven at 37°C and characterised by several methods such as Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TG), differential thermogravimetric analysis (DTG), differential thermal analyses (DTA) and gas adsorption analysis (BET). The released concentration of tetracycline hydrochloride was studied as a function of time, and it was measured by ultraviolet spectrophotometry in the tetracycline wavelength. The drug delivery profiles were reasonably consistent with a diffusion model analysis. In addition, we observed higher release rates for the freeze-dried compared to those dried in an oven at 37°C. This higher release can be attributed to larger pore size for the freeze-dried sample systems with tetracycline, which promoted more water penetration, improving the drug diffusion. The analysis of the solution obtained in the release tests using high-performance liquid chromatography- mass spectrometry (HPLC-MS) confirmed that tetracycline was being released.