The use of an adjuvant system improves innate and adaptive immune response when associated with a Leishmania (Viannia) braziliensis antigen in a vaccine candidate against L. (Leishmania) infantum infection.

dc.contributor.authorMathias, Fernando Augusto Siqueira
dc.contributor.authorOstolin, Thais Lopes Valentim Di Paschoale
dc.contributor.authorReis, Levi Eduardo Soares
dc.contributor.authorCardoso, Jamille Mirelle de Oliveira
dc.contributor.authorBrito, Rory Cristiane Fortes de
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorRoatt, Bruno Mendes
dc.contributor.authorVieira, Paula Melo de Abreu
dc.contributor.authorReis, Alexandre Barbosa
dc.date.accessioned2023-10-10T20:48:47Z
dc.date.available2023-10-10T20:48:47Z
dc.date.issued2023pt_BR
dc.description.abstractBackground: The adjuvants’ optimal dose and the administration route can directly influence the epitope recognition patterns and profiles of innate response. We aimed to establish the effect and the optimal dose of adjuvant systems for proposing a vaccine candidate to be employed with Leishmania (Viannia) braziliensis. Methods: We evaluated the adjuvants saponin (SAP), monophosphoryl lipid A (MPL) and resiquimod (R-848) isolated and combined as adjuvant systems in a lower dose corresponding to 25%, 33%, and 50% of each adjuvant total dose. Male outbred BALB/c mice were divided into 13 groups, SAP, MPL, and R-848 isolated, and the adjuvant systems SAP plus MPL (SM), SAP plus R-848 (SR), and MPL plus R-848 (MR). Results: SM50 increased levels of all chemokines analyzed and TNF production, while it presented an increased inflammatory cell infiltrate in the skin with macrophage recruitment. Thus, we proposed a vaccine candidate employing L. (V.) braziliensis antigen associated with the SM adjuvant system against experimental L. (Leishmania) infantum challenge. We observed a significant increase in the frequency of cells expressing the central and effector memory CD4+ T cells phenotype in immunized mice with the LBSM50. In the liver, there was a decreased parasite load when mice received LBSM50. Conclusions: When combined with L. (V.) braziliensis antigen, SM50 increases TNF and IFN-γ, which generates central and effector memory CD4+ T cells. Therefore, using an adjuvant system can promote an effective innate immune response with the potential to compose future vaccines.pt_BR
dc.identifier.citationMATHIAS, F. A. S. et al. The use of an adjuvant system improves innate and adaptive immune response when associated with a Leishmania (Viannia) braziliensis antigen in a vaccine candidate against L. (Leishmania) infantum infection. Vaccines, v. 11, n. 2, artigo 395, 2023. Disponível em: <https://www.mdpi.com/2076-393X/11/2/395>. Acesso em: 01 ago. 2023.pt_BR
dc.identifier.doihttps://doi.org/10.3390/vaccines11020395pt_BR
dc.identifier.issn2076-393X
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/17549
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: PDF do artigo.pt_BR
dc.subjectAdjuvantspt_BR
dc.subjectAdjuvant systempt_BR
dc.subjectSaponinpt_BR
dc.subjectMonophosphoryl lipid Apt_BR
dc.subjectResiquimodpt_BR
dc.titleThe use of an adjuvant system improves innate and adaptive immune response when associated with a Leishmania (Viannia) braziliensis antigen in a vaccine candidate against L. (Leishmania) infantum infection.pt_BR
dc.typeArtigo publicado em periodicopt_BR

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