DEFAR - Departamento de Farmácia

URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530

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2022 - 20232

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Autor: search.filters.author.Assis, Jéssica Vieira de

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    Rapid antigen test as a tool for the identification of SARS-CoV-2 infection and its potential as a self-testing device.
    (2023) Filgueiras, Priscilla Soares; Corsini, Camila Amormino; Almeida, Nathalie Bonatti Franco; Pedrosa, Maria Luysa Camargos; Miranda, Daniel Alvim Pena de; Gomes, Sarah Vieira Contin; Assis, Jéssica Vieira de; Silva, Raphael Antônio; Medeiros, Maria Izabella Vieira de Assis Rocha Carvalho de; Lourenço, Adelina Junia; Bicalho, Cecilia Maria Florencio; Vilela, Raquel Virgínia Rocha; Jeremias, Wander de Jesus; Fernandes, Gabriel da Rocha; Grenfell, Rafaella Fortini Queiroz
    Background: SARS-CoV-2 virus originated in Wuhan (China) in December (2019) and quickly spread worldwide. Antigen tests are rapid diagnostic tests (RDT) that produce results in 15-30 min and are an important tool for the scale-up of COVID-19 testing. COVID-19 diagnostic tests are authorized for self-testing at home in some countries, including Brazil. Widespread COVID-19 diagnostic testing is required to guide public health policies and control the speed of transmission and economic recovery. Methods: Patients with suspected COVID-19 were recruited at the Hospital da Baleia (Belo Horizonte, Brazil). The SARS-CoV-2 antigen- detecting rapid diagnostic tests were evaluated from June 2020 to June 2021 using saliva, nasal, and nasopharyngeal swab samples from 609 patients. Patient samples were simultaneously tested using a molecular assay (RT-qPCR). Sensitivity, specificity, accuracy, and positive and negative predictive values were determined using the statistical program, MedCalc, and GraphPad Prism 8.0. Results: The antigen-detecting rapid diagnostic tests displayed 98% specificity, 60% sensitivity, 96% positive predictive value, and moderate concordance with RT-qPCR. Substantial agreement was found between the two methods for patients tested < 7 days of symptom onset. Conclusions: Our findings support the use of Ag-RDT as a valuable and safe diagnostic method. Ag-RDT was also demonstrated to be an important triage tool for suspected COVID-19 patients in emergencies. Overall, Ag-RDT is an effective strategy for reducing the spread of SARS-CoV-2 and contributing to COVID-19 control.
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    Tetraspanin co029 expression as a tumor biomarker for monoclonal antibodies preparation : antigenic assessment in colorectal cancer cells.
    (2022) Coutinho, Lucelia; Corsini, Camila Amormino; Assis, Jéssica Vieira de; Pedrosa, Maria Luysa; Jeremias, Wander de Jesus; Santos, Viviane; Cabral, Mônica Maria Demas Álvares; Mesquita, Ana Sofia; Viviani, Matheus; Rodrigues, Angelica Nogueira; Grenfell, Rafaella Fortini Queiroz
    Introduction: The identification of innovative cancer biomarkers is a very relevant ongoing quest. Moreover, their role in cancer diagnosis and clinical management has been radically changed in the last few years with the major emphasis on cancer molecular classification, therapeutic target identification, and therapeutic protocol responsivity. tetraspanins are a family of transmembrane proteins correlated with tumor stage, tumor type and patient out- come affecting cell growth, morphology, invasion, and metastasis. Methods: We expressed the 31kDa transmembrane human tetraspanin co029 antigen in Escherichia coli expression host cells using Gateway® platform. Western blotting and ELISA techniques, together with gene sequencing, confirmed the identity of TSP co029 recombinant protein. Forty clones producing antibodies against TSP co029 were obtained. These antibodies were incubated with human colorectal cancer cells in different conditions. ELISA and immunohistochemistry were also performed. Results: The expressed tetraspanin had an appropriate conformation and antigenic integrity to produce antibodies with affinity to the native TSP co029 biomarker. The affinity of the purified recombinant protein and antibodies were confirmed by western blotting, florescent staining of human colorectal cancer cells in fluorescence and confocal microscopies and by ELISA and immunohistochemistry. Conclusion: Our data showed that the recombinant protein and antibodies produced in this study allowed the confirmation of tetraspanin co029 protein presented on the surface of human colorectal cancer cells.