DEALI - Departamento de Alimentos
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/540
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Item Pitanga and grumixama extracts : antioxidant and antimicrobial activities and incorporation into cellulosic films against Staphylococcus aureus.(2020) Carvalho, Franciele Mendes de; Martins, Jéssica Tatiana Aparecida; Lima, Emília Maria França; Santos, Hellen Vidal; Pereira, Patrícia Aparecida Pimenta; Pinto, Uelinton Manoel; Cunha, Luciana Rodrigues daThere is great interest in developing alternatives to improve food safety, since food-borne diseases (FBD) are a major public health concern worldwide. Plant extracts have the potential to inhibit microbial growth due to the action of secondary metabolites, such as phenolic compounds. This study evaluated the antioxidant and antimicrobial activities of phenolic compounds extracted from grumixama (Eugenia brasiliensis) and pitanga (Eugenia uniflora) fruits and the antimicrobial potential of extract of grumixama upon incorporation into cellulosic films. Both fruits were rich in total phenolic compounds and their extracts showed antioxidant activity. The crude and phenolic extracts of grumixama showed higher activity than those isolated from pitanga. All extracts inhibited the growth of Staphylococcus aureus. After incorporation into cellulosic films, the crude extract of grumixama remained active, reducing the S. aureus population in 4 log cycles. The cellulosic films incorporated with grumixama extract were stable after seven days of storage under refrigeration at 7º C; but they partially lost antimicrobial activity when exposed to UV radiation. These phenolic compound-containing cellulosic films could be used as a complementary preservation method of foods that are prone to contamination with S. aureus.Item Antioxidant, antimicrobial and anti - quorum sensing activities of Rubus rosaefolius phenolic extract.(2016) Oliveira, Brígida D’Ávila; Rodrigues, Adeline Conceição; Cardoso, Bárbara Moreira Inácio; Ramos, Ana Luiza Coeli Cruz; Bertoldi, Michele Corrêa; Taylor, Jason Guy; Cunha, Luciana Rodrigues da; Pinto, Uelinton ManoelPhenolic compounds are plant secondary metabolites that present many biological effects includingantioxidant and antimicrobial activities. Studies have shown that these compounds can also inhibitquorum sensing bacterial communication. The aim of this study was to characterize the centesimalcomposition, mineral and phenolic content, to determine the antioxidant and antimicrobial effect aswell as the inhibition of quorum sensing by the phenolic extract obtained from wild strawberry (Rubusrosaefolius). Centesimal composition and minerals were in the range expected for fruits of the Rubusgenerum, even though Fe and Zn presented higher levels. The phenolic content was 5902.89 mg GAE/L,also approaching the levels found for fruits of Rubus sp. The antioxidant activity determined throughthe ABTS method was 162.4 ± 5.6 and 120.8 ± 1.5 _M Trolox/g of fruit in the DPPH assay, indicating anelevated potential for ABTS and medium potential for DPPH method. The phenolic extract was able toinhibit all the evaluated bacteria presenting MICs in the range of 491.90–1475.74 mg GAE/L. In sub-MIC concentrations, the phenolic extract inhibited all the phenotypes typically regulated by quorum sens-ing in bacteria, including violacein production, swarming motility and biofilm formation. The phenolicextract of R. rosaefolius presented antioxidant, antimicrobial and anti-quorum sensing activities whichare in agreement with previous studies linking phenolic compounds to these properties.