DEALI - Departamento de Alimentos

URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/540

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Açai (Euterpe oleracea Mart.) dietary intake affects plasma lipids, apolipoproteins, cholesteryl ester transfer to high-density lipoprotein and redox metabolism : a prospective study in women.
(2016) Pala, Daniela; Barbosa, Priscila Oliveira; Silva, Carla Teixeira; Souza, Melina Oliveira de; Freitas, Fatima Rodrigues; Volp, Ana Carolina Pinheiro; Maranhão, Raul Cavalcante; Freitas, Renata Nascimento de
The açai fruit (Euterpe oleracea Martius), which is native to the Brazilian Amazon region, was shown to have high polyphenols and MUFA contents. In this study, we aimed to assess the effects of açai consumption on plasma lipids, apolipoproteins, the transfer of lipids to HDL (which is a relevant HDL function), and some biomarkers of redox metabolism. Forty healthy volunteer women aged 24 ± 3 years consumed 200 g of açai pulp/day for 4 weeks; their clinical variables and blood sample were obtained before and after this period. Açai pulp consumption did not alter anthropometric parameters, systemic arterial pressure, glucose, insulin and total, LDL and HDL cholesterol, triglycerides and apolipoprotein (apo) B, but it did increase the concentration of apo A-I. Açai consumption decreased the ROS, ox-LDL and malondialdehyde while increasing the activity of antioxidative paraoxonase 1. Overall, the total antioxidant capacity (TAC) was increased. Regarding the transfer of plasma lipids to HDL, açai consumption increased the transfer of cholesteryl esters (p ¼ 0.0043) to HDL. Unesterified cholesterol, phospholipids and triglyceride transfers were unaffected. The increase in apo A-I and the cholesteryl ester transfer to HDL after the açai intake period suggests that an improvement in the metabolism of this lipoprotein occurred, and it is well known that HDL is protective against atherosclerosis. Another important finding was the general improvement of the anti-oxidant defences elicited by açai consumption. Our data indicate that açai has favourable actions on plasma HDL metabolism and anti-oxidant defence; therefore açai could have a beneficial overall role against atherosclerosis, and it is a consistently good candidate to consider as a functional food.
Açaí (Euterpe oleracea Martius) promotes jejunal tissue regeneration by enhancing antioxidant response in 5-Fluorouracil-Induced mucositis.
(2020) Magalhães, Talita Alves Faria Martins; Souza, Melina Oliveira de; Gomes, Sttefany Viana; Silva, Raiana Mendes e; Martins, Flaviano dos Santos; Freitas, Renata Nascimento de; Amaral, Joana Ferreira do
Intestinal mucositis (IM) caused by antineoplastic chemotherapy is characterized by an important inflammatory process, which may compromise ongoing treatment. Our study aimed to investigate the effect of Ac¸aı (Euterpe oleracea Martius) on the antioxidant response in BALB/c mice pretreated with Ac¸aı pulp (200 g/kg) for 14 day. A group of animals receiving a single intraperitoneal injection of 5-FU (200 mg/kg) were euthanized on day three (D3) or seven (D7) after administration, the distal jejunum was isolated for the analyses of histology, superoxide dismutase (SOD) and catalase (CAT) enzyme activities, and concentration of total sulfhydryl groups (GSH). Seven days after induction, the intake of Ac¸aı by the IM group almost completely regenerated tissue histology. Notably, SOD activity decreased in the MUC þ Ac¸aı group (D3). CAT activity reduced on D3 and D7 in the IM groups and Ac¸aı treatment groups, respectively. No change was observed in the total GSH concentration at the tissue level. Our results demonstrated the protective effect of Ac¸aı pulp components on intestinal damage induced by 5-FU, as well as the ability to control the response to oxidative stress, in order to mobilize defense pathways and promote tissue repair.
Açaí (Euterpe oleracea Martius) supplementation improves oxidative stress biomarkers in liver tissue of dams fed a high-fat diet and increases antioxidant enzymes’ gene expression in offspring.
(2021) Barbosa, Priscila Oliveira; Souza, Melina Oliveira de; Silva, Maraísa Porfíria S.; Santos, Gabrielly Thaís dos; Silva, Marcelo Eustáquio; Bermano, Giovanna; Freitas, Renata Nascimento de
Lipids excess from an uterine environment can increase free radicals production of and thus induce oxidative status imbalance, a key factor for progression of non-alcoholic fatty liver disease (NAFLD) in offspring. Food antioxidant components in maternal diet may play an important role in preventing offspring metabolic disorders. The objective of the study was to evaluate the effects of açaí pulp supplementation on maternal high-fat diet, by assessing activity and expression of antioxidant enzymes and biomarkers of oxidative stress in the liver. Female Fisher rats were divided into four groups and fed a control diet (C), a high-fat diet (HF), a control diet supplemented with açaí (CA) and a high-fat diet supplemented with açaí (HFA) before mating, during gestation and lactation. The effects of açaí supplementation on oxidative stress biomarkers and antioxidant enzymes expression were evaluated in dams and male offspring after weaning. HFA diet increased body weight in dams, however reduced absolute and relative liver weight. There was a reduction in liver biomarkers of oxidative stress, malondialdehyde and carbonyl protein, as well as in catalase, glutathione peroxidase and superoxide dismutase activity. In offspring, HFA diet reduced liver weight and increased Gpx1, Gpx4 and Sod1 mRNA expression. These results suggest that açaí is able to restore redox status, preventing oxidative damage in dams by a direct mechanism and to promote beneficial effects on expression of antioxidant defences related genes in offspring.
Açaí (Euterpe oleracea Martius) supplementation in the diet during gestation and lactation attenuates liver steatosis in dams and protects ofspring.
(2019) Barbosa, Priscila Oliveira; Souza, Melina Oliveira de; Paiva, Deuziane Pereira Domingos de; Silva, Marcelo Eustáquio; Lima, Wanderson Geraldo de; Bermano, Giovanna; Freitas, Renata Nascimento de
Purpose: Maternal high-fat diet affects offspring and can induce metabolic disorders such as non-alcoholic fatty liver disease (NAFLD). New therapeutic strategies are being investigated as way to prevent or attenuate this condition. The objective of this study was to evaluate the effect of açaí supplementation in the maternal high-fat diet on dams and offspring lipid metabolism. Methods: Female Fisher rats were divided in four groups and fed a control diet (C), a high-fat diet (HF), an açaí supplemented diet (CA) and a high-fat diet supplemented with açaí (HFA) 2 weeks before mating, during gestation and lactation. The effects of açaí were evaluated in the male offspring after birth (P1) and weaning (P21). Results: HFA reduced relative liver weight, fat and cholesterol liver content in dams and improved liver steatosis as confirmed by histological analyses. HFA increased serum cholesterol and expression of Srebpf1 and Fasn genes. In offspring, HFA decreased relative liver weight, and serum cholesterol only in P21. An increase in the Sirt1, Srebpf1 and Fasn genes expression was observed in P21. Conclusions: These results suggest that açaí supplementation may attenuate NAFLD in dams and protect offspring from the detrimental effects of lipid excess from a maternal high-fat diet.
Açaí : an ally for human health?
(2018) Barbosa, Priscila Oliveira; Pala, Daniela; Souza, Melina Oliveira de; Freitas, Renata Nascimento de
Nutrition is one of the oldest sciences that aims to study the interactions between food, nutrients and human health. This fact can be checked from the famous citation of Hippocrates, considered the father of the modern medicine “Let food be thy medicine and medicine be thy food”. In the last decades several studies have attributed to foods the ability to provide benefits which go beyond its macronutrient content, being able to act on cellular signaling pathways reducing the incidence of many chronic diseases, including cardiovascular diseases (CVD) (1).
Acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis.
(2017) Almeida, Felipe Alves de; Pimentel Filho, Natan de Jesus; Carrijo, Lanna Clícia; Bento, Cláudia Braga Pereira; Pereira, Maria Cristina Baracat; Pinto, Uelinton Manoel; Oliveira, Leandro Licursi de; Vanetti, Maria Cristina Dantas
Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein. Our study aimed to evaluate the influence of AI-1 on the abundance of proteins and the levels of organic acids of Salmonella Enteritidis. The presence of N-dodecyl-homoserine lactone (C12- HSL) did not interfere on the growth or the total amount of extracted proteins of Salmonella. However, the abundance of the proteins PheT, HtpG, PtsI, Adi, TalB, PmgI (or GpmI), Eno, and PykF enhanced while the abundance of the proteins RplB, RplE, RpsB, Tsf, OmpA, OmpC, OmpD, and GapA decreased when Salmonella Enteritidis was anaerobically cultivated in the presence of C12-HSL. Additionally, the bacterium produced less succinic, lactic, and acetic acids in the presence of C12-HSL. However, the concentration of extracellular formic acid reached 20.46 mM after 24 h and was not detected when the growth was in the absence of AI-1. Considering the cultivation period for protein extraction, their abundance, process and function, as well as the levels of organic acids, we observed in cells cultivated in presence of C12-HSL a correlation with what is described in the literature as entry into the stationary phase of growth, mainly related to nitrogen and amino acid starvation and acid stress. Further studies are needed in order to determine the specific role of the differentially abundant proteins and extracellular organic acids secreted by Salmonella in the presence of quorum sensing signaling molecules.
Acyl homoserine lactone‑based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions.
(2017) Almeida, Felipe Alves de; Pimentel Filho, Natan de Jesus; Pinto, Uelinton Manoel; Mantovani, Hilário Cuquetto; Oliveira, Leandro Licursi de; Vanetti, Maria Cristina Dantas
Quorum sensing regulates a variety of phenotypes in bacteria including the production of virulence factors. Salmonella spp. have quorum sensing systems mediated by three autoinducers (AI-1, AI-2, and AI-3). The AI-1-mediated system is incomplete in that the bacterium relies on the synthesis of signaling molecules by other microorganisms. This study aimed to evaluate the influence of the AI-1 N-dodecanoyl-DL-homoserine lactone (C12- HSL) on the growth, motility, adhesion, and biofilm formation of Salmonella enterica serovar Enteritidis PT4 578 on a polystyrene surface. Experiments were conducted at 37 °C in anaerobic tryptone soy broth supplemented with C12-HSL and/or a mixture of four synthetic furanones, at the concentration of 50 nM each. The planktonic growth, adhesion, swarming, and twitching motility were not altered in the presence of C12-HSL and/or furanones under anaerobic conditions. However, C12-HSL induced biofilm formation after 36 h of cultivation as determined by quantification of biofilm formation, by enumeration of adhered cells to polystyrene coupons, and finally by imaging the presence of multilayered cells on an epifluorescence microscope. When furanones were present in the medium, an antagonistic effect against C12-HSL on the biofilm development was observed. The results demonstrate an induction of biofilm formation in Salmonella Enteritidis by AI-1 under anaerobic conditions. Considering that Salmonella does not produce AI-1 but respond to it, C12-HSL synthesized by other bacterial species could trigger biofilm formation by this pathogen in conditions that are relevant for its pathogenesis.
Adição de fontes de nitrogênio e de duas linhagens de levedura na fermentação alcoólica para produção de cachaça.
(2015) Pereira, Alexandre Fontes; Silva, Paulo Henrique Alves da; Pinheiro, Patrícia Fontes; Pinheiro, Carlos Alexandre; Braga, Leonel Martins
Neste trabalho foi avaliada a influência de duas linhagens de levedura (baixa fermentação (Saflager) e alta fermentação (Safbrew)) e três fontes de nitrogênio (sulfato de amônio, peptona de caseína e um pool de aminoácidos) nas concentrações 5 g.L-1 e 25 g.L-1, na composição do mosto durante a fermentação para produção da cachaça. As propriedades físico-químicas avaliadas foram: pH, acidez total, teor de sólidos solúveis e teor alcoólico, em amostras coletadas em 12, 24, 48, 72 e 96 horas de fermentação. As amostras de cachaça produzidas foram analisadas por cromatografia a gás, sendo que os alcoóis superiores, propan-1-ol, álcool isobutílico e álcool isoamílico tiveram comportamentos diferentes para as linhagens de leveduras de baixa fermentação e alta fermentação. A maior produção de etanol por consumo de substrato foi obtido pela linhagem de levedura Saflager (baixa fermentação) na fonte de nitrogênio sulfato de amônio, na concentração de 5 g.L-1, nos mostos de cachaça.
Adoptive transfer of dendritic cells expressing CD11c reduces the immunological response associated with experimental colitis in BALB/c mice.
(2018) Paiatto, Lisiery Negrini; Silva, Fernanda Guimarães Drummond e; Yamada, Aureo Tatsumi; Tamashiro, Wirla Maria da Silva Cunha; Simioni, Patricia Ucelli
Introduction In addition to conventional therapies, several new strategies have been proposed for modulating autoimmune diseases, including the adoptive transfer of immunological cells. In this context, dendritic cells (DCs) appear to be one of the most promising treatments for autoimmune disorders. The present study aimed to evaluate the effects of adoptive transfer of DCs obtained from both naïve and ovalbumin (OVA)-tolerant mice on the severity of TNBS induced colitis and analyze the eventual protective mechanisms. Methods and results To induce oral tolerance, BALB/c mice were fed 4mg/mL OVA solution for seven consecutive days. Spleen DCs were isolated from tolerant (tDC) and naïve (nDC) mice, and then adoptively transferred to syngeneic mice. Three days later, colitis was induced in DC treated mice by intrarectal instillation of 100μg2,4,6-trinitrobenzenesulfonic acid (TNBS) dissolved in 50% ethanol. Control subjects received only intrarectal instillation of either TNBS solution or a vehicle. Five days later, mice from all groups were euthanized and examined for physiological and immunological parameters. Regarding the phenotype, we observed that the frequencies of CD11+ MHC II+ and CD11+ MHCII+ CD86+ cells were significantly lower in DCs isolated from tolerant mice than in those from naive mice. However, pretreatment with both types of DCs was able to significantly reduce clinical signs of colitis such as diarrhea, rectal prolapse, bleeding, and cachexia, although only treatment with tDCs was able to prevent weight loss from instillation of TNBS. In vitro proliferation of spleen cells from mice treated with either type of DCs was significantly lower than that observed in splenic cell cultures of naïve mice. Although no significant difference was observed in the frequencies of Treg cells in the experimental groups, the frequency of Th17+CD4+cellsand the secretion of IL-17 were more reduced in the cultures of spleen cells from mice treated with either type of DCs. The levels of IL-9 and IFN-γ were lower in supernatants of cells from mice treated with nDCs. Conclusion The results allow us to conclude that the adoptive transfer of cells expressing CD11c is able to reduce the clinical and immunological signs of drug-induced colitis. Adoptive transfer of CD11c+DC isolated from both naive and tolerant mice altered the proliferative and T cell responses. To the best of our knowledge, there is no previously published data showing the protective effects of DCs from naïve or tolerant mice in the treatment of colitis.
Amphiphilic niobium oxyhydroxide as a hybrid catalyst for sulfurremoval from fuel in a biphasic system.
(2014) Oliveira, Luiz Carlos Alves de; Costa, Nathália Tavares; Pliego Junior, Josefredo Rodriguez; Silva, Adilson Cândido da; Souza, Patterson Patrício de; Patrício, Patrícia Santiago de Oliveira
Here, we show that a new niobium oxyhydroxide, NbO2OH, can be synthesized and modified with asurfactant to be used as a heterogeneous catalyst for oxidative desulphurization. The material was treatedwith hydrogen peroxide to generate oxidizing groups (peroxo species) on the surface. Furthermore, thismaterial was converted to a family of solid catalysts that can stabilize water/oil emulsions and catalyzereactions at the liquid/liquid interface by anchoring a surfactant to the catalyst. The material was thenable to efficiently remove a sulfurous compound under mild conditions.
Análise de características físicas e sensoriais de bolos de abacaxi com substituição e/ou redução de sacarose.
(2021) Mendes, Elaine Cristina; Mota, Bruna Eugênia Ferreira; Vasconcelos, Christiane Mileib; Pereira, Patrícia Aparecida Pimenta; Cunha, Simone de Fátima Viana da
A sacarose é um importante contribuinte para o valor calórico total de um alimento, além de ser um nutriente muito relevante para algumas patologias, como diabetes mellitus. Esse estudo objetivou reformular uma preparação de bolo de abacaxi, reduzindo e/ou substituindo o teor de açúcar. Uma receita de bolo de abacaxi foi escolhida, padronizada e receitas com modificações foram confeccionadas. Elaborou-se 8 formulações, sendo: B1-Padrão; B2-Padrão com redução de 50 % de açúcar cristal; B3-Açúcar mascavo; B4-Açúcar mascavo com redução de 50%; B5- Açúcar light; B6-Açúcar light com redução de 50%; B7- Edulcorante e B8- Edulcorante com redução de 50%. Posteriormente foram realizadas análises física e sensorial das preparações. Uma maior densidade específica da massa crua foi observada em B5 e uma menor densidade em B8. Em relação ao volume específico dos bolos, as maiores médias foram encontradas em B1, B7 e B8. As formulações B1, B2 e B6 obtiveram uma maior aceitação em relação aos atributos, doçura, consistência, aparência e impressão global. Com a redução em 50% ou substituição do açúcar pelo adoçante, como visto em B2 e B6, respectivamente, não houve alteração estatisticamente significativa nas características físicas delas, tampouco foi fator de rejeição. Dessa forma, possibilita-se o desenvolvimento de formulações de bolos de abacaxi, com redução e/ou substituição do açúcar sem perder suas propriedades sensoriais e físicas de volume, de forma a garantir sua aceitabilidade.
Analysis of the subtropical blackberry cultivar potential in jelly processing.
(2014) Souza, Vanessa Rios de; Pereira, Patrícia Aparecida Pimenta; Pinheiro, Ana Carla Marques; Lima, Luiz Carlos de Oliveira; Pio, Rafael; Queiroz, Fabiana
In Brazil, there are numerous blackberry cultivars under cultivation, however, only a few cultivars, such as the Guarani, are displayed/used for processing. In this context the aim of this work was to study the effects of different Brazilian blackberry cultivars (Guarani, Brazos, Comanche, Tupy, Cherokee, Caingangue, and Choctaw) on the physicochemical characteristics, texture profiles and the consumer acceptance of the resulting jelly to identify the potential use of these cultivars in the jelly industry. It is feasible to produce blackberry jellies with the Tupy, Comanche, Brazos, Guarani, and Choctaw cultivars because these jellies demonstrated good acceptability combined with good productivity. Consumers were shown to have a preference for a less adhesive, more acidic jelly. This study provides a useful insight about the Brazilian blackberry cultivar potential in jelly processing.
Analysis of various sweeteners in low-sugar mixed fruit jam : equivalent sweetness, time-intensity analysis and acceptance test.
(2013) Souza, Vanessa Rios de; Pereira, Patrícia Aparecida Pimenta; Pinheiro, Ana Carla Marques; Bolini, Helena Maria André; Borges, Soraia Vilela; Queiroz, Fabiana
For a sweetener to successfully replace sucrose in food formulations, studies must first be conducted to determine the concentrations of the sweeteners to be used and their equivalent sweetness compared with sucrose. After establishing the optimal concentration of each sweetener, it is necessary to determine which is more similar to sucrose. The objective of this study was to determine the equivalent amount of different sweeteners, necessary to promote the same degree of ideal sweetness in mixed fruit (marolo, sweet passion fruit and soursop) jam and to characterise the time–intensity profile and consumer acceptance. With respect to the mixed fruit jam containing 40% (w/w) of sucrose, sucralose presented the highest sweetening power, being 1033.59 times sweeter than sucrose, followed by sucralose/acesulfame-K/neotame 5:3:0.1 (982.80), sucralose/steviol glycoside 2:1 (862.67), sucralose/acesulfame-K 3:1 (847.45) and sucralose/thaumatin 1:0.6 (284.29). The sweeteners had a time–intensity sweetness profile similar to sucrose and a time– intensity bitterness profile different from sucrose but similar among themselves. In relation to sensory acceptance, a significant difference between the low-sugar jam and the traditional jam was not observed.
Animal models for the study of arterial hypertension.
(2011) Dornas, Waleska Claudia Amaral; Silva, Marcelo Eustáquio
Hypertension is one of the leading causes of disability or death due to stroke, heart attack and kidney failure. Because the etiology of essential hypertension is not known and may be multifactorial, the use of experimental animal models has provided valuable information regarding many aspects of the disease, which include etiology, pathophysiology, complications and treatment. The models of hypertension are various, and in this review, we provide a brief overview of the most widely used animal models, their features and their importance.
Annato extract and β-carotene modulate the production of reactive oxygen species/nitric oxide in neutrophils from diabetic rats.
(2012) Rossoni Júnior, Joamyr Victor; Araújo, Glaucy Rodrigues de; Pádua, Bruno da Cruz; Chaves, Míriam Martins; Pedrosa, Maria Lúcia; Silva, Marcelo Eustáquio; Costa, Daniela Caldeira
Annatto has been identified asecarotenoids that havetantioxidative effects. It is well known that one of the key elements in the development of diabetic complications is oxidative stress. The immune system is especially vulnerable to oxidative damage because many immune cells, such as neutrophils, produce reactive oxygen species and reactive nitrogen species as part of the body’s defense mechanisms to destroy invading pathogens. Reactive oxygen species/reactive nitrogen species are excessively produced by active peripheral neutrophils, and may damage essential cellular components, which in turn can cause vascular complications in diabetes. The present study was undertaken to evaluate the possible protective effects of annatto on the reactive oxygen species and nitric oxide (NO) inhibition in neutrophils from alloxan-induced diabetic rats. Adult female rats were divided into six groups based on receiving either a standard diet with or without supplementation of annatto extract or beta carotene. All animals were sacrificed 30 days after treatment and the neutrophils were isolated using two gradients of different densities. The reactive oxygen species and NO were quantified by a chemiluminescence and spectrophotometric assays, respectively. Our results show that neutrophils from diabetic animals produce significantly more reactive oxygen species and NO than their respective controls and that supplementation with beta carotene and annatto is able to modulate the production of these species. Annatto extract may have therapeutic potential for modulation of the balance reactive oxygen species/NO induced by diabetes.
Anthropometric risk factors for metabolic syndrome in HIV patients.
(2016) Leal, José Adalberto; Fausto, Maria Arlene; Carneiro, Mariângela
OBJECTIVE: Metabolic syndrome, which affects the general population in epidemic proportions, is associated with a set of cardiovascular disease risk factors. The aims of this cross-sectional study were to determine the prevalence and investigate the risk factors associated with metabolic syndrome in outpatients living with HIV/AIDS using anthropometric and clinical evaluations. METHOD: The study was carried out on 253 HIV infected outpatients. Metabolic syndrome was classified according to the National Cholesterol Education Program Adult Treatment Panel III (NCEP/ATPIII) and the International Diabetes Federation (IDF) criteria. Logistic regression was used to identify factors associated with the metabolic syndrome. RESULTS: The prevalence of metabolic syndrome varied from 19.4% to 26.4%, according to the criterion used. The factors associated with it in the two classifications used, when adjusted by sex and BMI, were age (≥ 40years) and subscapular skinfold (> 12 mm). In the final model, using the NCEP/ATPIII criterion the risk factors associated with metabolic syndrome were age ≥ 40 years (OR = 3.18; CI95% = 1.42; 7.14) and subscapular skinfold > 12 mm (OR = 2.85, CI95% = 1.13; 7.17). In the final model, using the IDF criterion the risk factors associated with metabolic syndrome were age (OR = 3.38, CI95% = 1.61; 7.10) and subscapular skinfold > 12 mm (OR = 4.37, CI95% = 1.84; 10.39). CONCLUSION: In clinical practice, the regular monitoring of subscapular skinfold can help in the identification of HIV infected individuals in risk of MS.
Antimicrobial and antioxidant activity of apricot (Mimusopsis comersonii) phenolic-rich extract and its application as an edible coating for fresh-cut vegetable preservation.
(2022) Lima, Emília Maria França; Matsumura, Caroline Harumi Silva; Silva, Geovana Luísa da; Patrocínio, Isabela Cristina Soares; Santos, Catarina Angeli; Pereira, Patrícia Aparecida Pimenta; Hassimotto, Neuza Mariko Aymoto; Pinto, Uelinton Manoel; Cunha, Luciana Rodrigues da
Edible coatings have several advantages in preserving foods, such as avoiding water loss, controlling microbial growth, and reducing the need for preservatives added directly to the product. Antimicrobial action can be obtained by adding antimicrobial substances including phenolic compounds commonly found in plant extracts. This study evaluated the phenolic compounds content, antioxidant and antimicrobial activity of pulp, and seed extracts of Mimusopsis comersonii (popularly known in Brazil as abrico), besides the phenolic compounds were identified and quantified in the pulp extract. Edible coatings were incorporated with pulp extract in order to evaluate the preservation of minimally processed apples and baroa potatoes against foodborne bacteria, and enzymatic browning was also determined. Myricetin-3-glucoside, quercetin-3-glucoside, and kaempferol-3-glucoside were identified as major flavonoids in the apricot pulp extract. The seed and pulp extracts inhibited all tested microorganisms, especially Staphylococcus aureus and Salmonella Typhimurium. Edible coatings added with 9% of phenolic extract showed in vitro antimicrobial activity, in addition to being effective in preventing enzymatic browning in minimally processed apples and baroa potatoes for up to 15 days of storage. They were also effective in reducing up to 2 log CFU/g of aerobic mesophiles after 15 days of storage for apples, even though no microbial inhibition in baroa potatoes was observed under the same conditions. The addition of pulp phenolic extract in edible coatings proved to be an alternative in the preservation of apples and in the antibrowning activity of minimally processed baroa potatoes.
Antioxidant activity, sensory analysis and acceptability of red fruit juice supplemented with Brazilian green propolis.
(2022) Lopes, Géssica Aparecida; Fidelis, Priscila Cardoso; Almeida, Bruno de Moura; Almeida, Jane José; Ientz, Gislaine de Almeida Santana; Binda, Nancy Scardua; Teixeira, Arthur Ferrari; Vieira Filho, Sidney Augusto; Caligiorne, Rachel Basques; Guimarães, Dênia Antunes Saúde; Brumano, Maria Helena Nasser; Figueiredo, Sônia Maria de
In this work were determining the total phenolic content, total flavonoids and antioxidant capacity in formulations of red fruit juice supplemented with different concentrations of aqueous extract of Brazilian green propolis. And, also was investigate the formulation sensory acceptance by volunteers’ consumers. The addition of propolis to red fruit juice increased the content of total phenolic compounds and flavonoids, enhanced the antioxidant activity of the final product as assessed by radical scavenging DPPH and ABTS and FRAP methods, and no negative effect on consumer taste. All red fruit juice formulations containing propolis presented significant results through overall acceptability test and were approved in relation to purchase intent by the volunteer consumers. Formulation F3 (6.1 mg.mL-1) showed the best results. The antioxidant activity increment was proportional to the concentration of aqueous green propolis extracts added to the juice. The positive purchase intention reached up 64.75% of participants’ acceptance. A new, sugar-free drink with pleasurable sensory and functional properties, combined with health benefits, has been obtained.
Antioxidant biomarkers and food intake in elderly women.
(2012) Boaventura, Brunna Cristina Bremer; Pietro, Patrícia Faria Di; Assis, Maria Alice Altenburg de; Ambrosi, Cláudia; Nesello, Luciane Angela Nottar; Silva, Fabiana Ourique da; Vasconcelos, Francisco de Assis Guedes de; Moreira, José Claudio Fonseca; Fausto, Maria Arlene
To investigate the relationship between antioxidant biomarkers and food intake in elderly women. Design: Cross-sectional study. Setting: Recreation Center for the Elderly in the city of Itajaí, Santa Catarina, Brazil. Participants: 73 elderly women with an average age of 71 years, 93% caucasian, average body weight 68.7 ± 13.1 kg and average BMI 28.5 ± 2.3 kg/m2.
Antioxidant, antimicrobial and anti - quorum sensing activities of Rubus rosaefolius phenolic extract.
(2016) Oliveira, Brígida D’Ávila; Rodrigues, Adeline Conceição; Cardoso, Bárbara Moreira Inácio; Ramos, Ana Luiza Coeli Cruz; Bertoldi, Michele Corrêa; Taylor, Jason Guy; Cunha, Luciana Rodrigues da; Pinto, Uelinton Manoel
Phenolic compounds are plant secondary metabolites that present many biological effects includingantioxidant and antimicrobial activities. Studies have shown that these compounds can also inhibitquorum sensing bacterial communication. The aim of this study was to characterize the centesimalcomposition, mineral and phenolic content, to determine the antioxidant and antimicrobial effect aswell as the inhibition of quorum sensing by the phenolic extract obtained from wild strawberry (Rubusrosaefolius). Centesimal composition and minerals were in the range expected for fruits of the Rubusgenerum, even though Fe and Zn presented higher levels. The phenolic content was 5902.89 mg GAE/L,also approaching the levels found for fruits of Rubus sp. The antioxidant activity determined throughthe ABTS method was 162.4 ± 5.6 and 120.8 ± 1.5 _M Trolox/g of fruit in the DPPH assay, indicating anelevated potential for ABTS and medium potential for DPPH method. The phenolic extract was able toinhibit all the evaluated bacteria presenting MICs in the range of 491.90–1475.74 mg GAE/L. In sub-MIC concentrations, the phenolic extract inhibited all the phenotypes typically regulated by quorum sens-ing in bacteria, including violacein production, swarming motility and biofilm formation. The phenolicextract of R. rosaefolius presented antioxidant, antimicrobial and anti-quorum sensing activities whichare in agreement with previous studies linking phenolic compounds to these properties.