EFAR - Escola de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/451
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O curso de Farmácia em Ouro Preto foi criado em 1839, sendo a mais antiga Escola de Farmácia da América Latina.
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Item Chemical constituents, antioxidant potential, antibacterial study and photoprotective activity of Brazilian corn silk extract.(2022) Azevedo, Amanda Scofield de; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Antunes, Amanda dos Santos; Sousa, Lucas Resende Dutra; Souza, Gustavo Henrique Bianco de; Teixeira, Luiz Fernando de Medeiros; Vieira, Paula Melo de Abreu; Santos, Viviane Martins Rebello dos; Nascimento, Adriana Mendes do; Nascimento, Andréa Mendes doThe aim of this work was to determine the total phenolic content and evaluate the antioxidant, antibacterial, photoprotective and cytotoxic properties of corn silk ethanol extract prepared from corn silk that was air-dried at room temperature. Corn silk ethanol extract has a high content of phenolic compounds. The concentrations of ethanol extract required to produce 50% of maximal effect (EC50) in DPPH and ABTS radical scavenging assays were 489.0 and 166.1 μg/mL respectively, indicating low activity when compared to the positive control. The ethanolic extract of corn silk showed antibacterial activity, mainly on Gram-positive bacteria. The in vitro sun protection factor (SPF) of the ethanolic extract of corn silk incorporated into sunscreen UVA-UVB 5% gel with Pemulen TR-1® was evaluated for the first time. A relative increase in SPF efficacy was observed for the extract when the sunscreen was incorporated. The ethanolic extract of corn silk and the gel were considered non-cytotoxic. The corn silk ethanolic extract was characterized using ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS). Eleven compounds including flavonoids, coumaric acid derivatives, terpenoid, hydroxycinnamic acid derivative and quinic acid derivative were suggested based on their accurate mass and MS/MS spectra.Item The use of an adjuvant system improves innate and adaptive immune response when associated with a Leishmania (Viannia) braziliensis antigen in a vaccine candidate against L. (Leishmania) infantum infection.(2023) Mathias, Fernando Augusto Siqueira; Ostolin, Thais Lopes Valentim Di Paschoale; Reis, Levi Eduardo Soares; Cardoso, Jamille Mirelle de Oliveira; Brito, Rory Cristiane Fortes de; Soares, Rodrigo Dian de Oliveira Aguiar; Roatt, Bruno Mendes; Vieira, Paula Melo de Abreu; Reis, Alexandre BarbosaBackground: The adjuvants’ optimal dose and the administration route can directly influence the epitope recognition patterns and profiles of innate response. We aimed to establish the effect and the optimal dose of adjuvant systems for proposing a vaccine candidate to be employed with Leishmania (Viannia) braziliensis. Methods: We evaluated the adjuvants saponin (SAP), monophosphoryl lipid A (MPL) and resiquimod (R-848) isolated and combined as adjuvant systems in a lower dose corresponding to 25%, 33%, and 50% of each adjuvant total dose. Male outbred BALB/c mice were divided into 13 groups, SAP, MPL, and R-848 isolated, and the adjuvant systems SAP plus MPL (SM), SAP plus R-848 (SR), and MPL plus R-848 (MR). Results: SM50 increased levels of all chemokines analyzed and TNF production, while it presented an increased inflammatory cell infiltrate in the skin with macrophage recruitment. Thus, we proposed a vaccine candidate employing L. (V.) braziliensis antigen associated with the SM adjuvant system against experimental L. (Leishmania) infantum challenge. We observed a significant increase in the frequency of cells expressing the central and effector memory CD4+ T cells phenotype in immunized mice with the LBSM50. In the liver, there was a decreased parasite load when mice received LBSM50. Conclusions: When combined with L. (V.) braziliensis antigen, SM50 increases TNF and IFN-γ, which generates central and effector memory CD4+ T cells. Therefore, using an adjuvant system can promote an effective innate immune response with the potential to compose future vaccines.Item ASP-2/Trans-sialidase chimeric protein induces robust protective immunity in experimental models of chagas’ disease.(2023) Castro, Júlia Teixeira de; Brito, Rory Cristiane Fortes de; Souza, Natália Satchiko Hojo de; Azevedo, Bárbara Ribeiro Batista Vaz de; Castro, Natália Salazar de; Ferreira, Camila Pontes; Giusta, Caroline Junqueira; Fernandes, Ana Paula Salles Moura; Vasconcellos, José Ronnie Carvalho de; Cardoso, Jamille Mirelle de Oliveira; Soares, Rodrigo Dian de Oliveira Aguiar; Vieira, Paula Melo de Abreu; Carneiro, Cláudia Martins; Valiate, Bruno Vinícius Santos; Toledo, Cristiane; Salazar, Andres M.; Caballero, Otávia; Vieira, Joseli Lannes; Teixeira, Santuza Maria Ribeiro; Reis, Alexandre Barbosa; Gazzinelli, Ricardo TostesImmunization with the Amastigote Surface Protein-2 (ASP-2) and Trans-sialidase (TS) antigens either in the form of recombinant protein, encoded in plasmids or human adenovirus 5 (hAd5) confers robust protection against various lineages of Trypanosoma cruzi. Herein we generated a chimeric protein containing the most immunogenic regions for T and B cells from TS and ASP-2 (TRASP) and evaluated its immunogenicity in comparison with our standard protocol of heterologous prime-boost using plasmids and hAd5. Mice immunized with TRASP protein associated to Poly-ICLC (Hiltonol) were highly resistant to challenge with T. cruzi, showing a large decrease in tissue parasitism, parasitemia and no lethality. This protection lasted for at least 3 months after the last boost of immunization, being equivalent to the protection induced by DNA/hAd5 protocol. TRASP induced high levels of T. cruzispecific antibodies and IFNγ-producing T cells and protection was primarily mediated by CD8+ T cells and IFN-γ. We also evaluated the toxicity, immunogenicity, and efficacy of TRASP and DNA/hAd5 formulations in dogs. Mild collateral effects were detected at the site of vaccine inoculation. While the chimeric protein associated with Poly-ICLC induced high levels of antibodies and CD4+ T cell responses, the DNA/hAd5 induced no antibodies, but a strong CD8+ T cell response. Immunization with either vaccine protected dogs against challenge with T. cruzi. Despite the similar efficacy, we conclude that moving ahead with TRASP together with Hiltonol is advantageous over the DNA/hAd5 vaccine due to pre-existing immunity to the adenovirus vector, as well as the cost-benefit for development and large-scale production.Item LBMPL vaccine therapy induces progressive organization of the spleen microarchitecture, improved Th1 adaptative immune response and control of parasitism in Leishmania infantum naturally infected dogs.(2022) Roatt, Bruno Mendes; Cardoso, Jamille Mirelle de Oliveira; Reis, Levi Eduardo Soares; Moreira, Gabriel José Lucas; Gonçalves, Letícia Captein; Marques, Flávia de Souza; Moreira, Nádia das Dores; Vieira, Paula Melo de Abreu; Soares, Rodrigo Dian de Oliveira Aguiar; Giunchetti, Rodolfo Cordeiro; Reis, Alexandre BarbosaThe spleen plays a central role in human and canine visceral leishmaniasis, where the activation of the immune response occurs in one of the tissues where Leishmania infantum reproduces. Therefore, this organ is both a target to understand the mechanisms involved in the parasite control and a parameter for assessing the therapeutic response. In this sense, this study aimed to evaluate the main histological, immunological and parasitological aspects in the spleen of symptomatic dogs naturally infected by L. infantum treated with the therapeutic vaccine LBMPL. For this, dogs were divided into four groups: dogs uninfected and untreated (NI group); L. infantum-infected dogs that were not treated (INT group); L. infantum-infected dogs that received treatment only with monophosphoryl lipid A adjuvant (MPL group); and L. infantum-infected dogs that received treatment with the vaccine composed by L. braziliensis promastigote proteins associated with MPL adjuvant (LBMPL group). Ninety days after the therapeutics protocol, the dogs were euthanized and the spleen was collected for the proposed evaluations. Our results demonstrated a reduction of hyperplasia of red pulp and follicular area of white pulp, increased mRNA expression of IFN-γ, TNF-α, IL-12 and iNOS, and decreased IL-10 and TGF-β1, and intense reduction of splenic parasitism in dogs treated with the LBMPL vaccine. These results possibly suggest that the pro-inflammatory environment promoted the progressive organization of the splenic architecture favoring the cellular activation, with consequent parasite control. Along with previously obtained data, our results propose the LBMPL vaccine as a possible treatment strategy for canine visceral leishmaniasis (CVL).Item Performance of the Wondfo 2019-nCoV antigen test using self-collected nasal versus professional-collected nasopharyngeal swabs in symptomatic SARS-CoV-2 infection.(2022) Cardoso, Jamille Mirelle de Oliveira; Roatt, Bruno Mendes; Vieira, Paula Melo de Abreu; Paiva, Nívia Carolina Nogueira de; Souza, Breno Bernardes; Lisboa, Oscar Campos; Soares, Rodrigo Dian de Oliveira Aguiar; Reis, Alexandre Barbosa; Vital, Wendel Coura; Carneiro, Cláudia MartinsItem Down regulation of IL-10 and TGF-β1 mRNA expression associated with reduced inflammatory process correlates with control of parasitism in the liver after treating L. infantum infected dogs with the LBMPL vaccine therapy.(2022) Roatt, Bruno Mendes; Cardoso, Jamille Mirelle de Oliveira; Brito, Rory Cristiane Fortes de; Reis, Levi Eduardo Soares; Moreira, Gabriel José Lucas; Vieira, Paula Melo de Abreu; Souza, Flávia Marques de; Lima, Wanderson Geraldo de; Soares, Rodrigo Dian de Oliveira Aguiar; Giunchetti, Rodolfo Cordeiro; Reis, Alexandre BarbosaThe liver plays an important role in human and canine visceral leishmaniasis, then it is considered as target to understand the mechanisms involved in the parasite control and a parameter to assess therapeutic responses. In this sense, our study focuses on evaluating the major alterations in the liver by histological (morphometric parenchyma inflammation/semi-quantitative portal inflammation), immunohistochemical assays (parasitism), and qPCR (parasitism and cytokine gene expression) in Leishmania infantum naturally infected dogs and treated with LBMPL vaccine. Animals were divided in four groups: NI group (n = 5): uninfected and untreated dogs; INT group (n = 7): L. infantum-infected dogs and not treated; MPL group (n = 6): L. infantum-infected dogs that received only monophosphoryl lipid A adjuvant, and LBMPL group (n = 10): L. infantum-infected dogs that received treatment with the vaccine composed by L. braziliensis disrupted promastigotes associated with MPL adjuvant. Ninety days after the end of treatments, the dogs were euthanized, and the liver was collected for the proposed evaluations. Significantly lower portal inflammatory reactions, and lower parenchyma inflammation were observed in the LBMPL group compared to INT and MPL groups. iNOS mRNA expression was higher in LBMPL group and in contrast, IL-10 and TGF-β1 mRNA expression was lower in this group when compared to INT group. Immunohistochemical and qPCR analysis showed significant parasite load reduction in LBMPL group compared to INT and MPL animals. Our data suggest that in naturally Leishmania-infected dogs, LBMPL vaccine reduces the damage in the hepatic tissue, being able to attenuate the type 2 immune response. It could be associated with a marked reduction in the parasitism decreasing liver inflammation in treated dogs. Along with previously obtained data, our results suggest that LBMPL vaccine can significantly contribute to the therapy strategy for L. infantum infected dogs.Item Cell immune response in mice skin stimulated with different adjuvants by intradermal route.(2022) Mathias, Fernando Augusto Siqueira; Cardoso, Jamille Mirelle de Oliveira; Reis, Levi Eduardo Soares; Souza, Juliana Vitoriano de; Moreira, Nádia das Dores; Ostolin, Thais Lopes Valentim Di Paschoale; Brito, Rory Cristiane Fortes de; Soares, Rodrigo Dian de Oliveira Aguiar; Vieira, Paula Melo de Abreu; Carneiro, Cláudia Martins; Roatt, Bruno Mendes; Reis, Alexandre BarbosaAdjuvants act in the innate immunity and, when combined to vaccine antigens, can produce a more intense response, improving the antigen presentation, directing the immune system, excellent for new vaccine formulations. This study evaluated the use of the intradermal route and the immune response triggered by a single dose of the adjuvants Aluminum Hydroxide (Al(OH)3 ), Montanide Pet Gel A (MPGA), Glucopyranosyl Lipid A Stable Emulsion (GLA-SE), and Resiquimod (R-848) in the mice skin. As control mice received sterile saline. MPGA and GLA-SE led to cell recruitment when compared with control group, with intense presence of neutrophils in first 12 hours, replaced by macrophages after 168 hours. R-848 and Al(OH)3 showed similar cell recruitment profiles. Regarding cytokine production, groups that received MPGA and GLA-SE produced high levels of IL-6, TNF-α, and IFN-γ. R-848 and Al(OH)3 groups displayed similar profile of cytokine production only at the first hour. Our results suggest that the intradermal route is efficient inducing immune system activation and GLA-SE was promising adjuvants for a type 1 immune response vaccine.Item New studies in vitro antibacterial evaluation and cell viability in PMMA-G-PEG 4000 derivatives with encapsulated Erythromycin.(2022) Azevedo, Maria Luíza Schaefer; Rezende, Giselle A. S.; Sousa, Lucas Resende Dutra; Reis, Rúbia Castro Fernandes Melo; Novack, Kátia Monteiro; Barboza, Ana Paula Moreira; Neves, Bernardo Ruegger Almeida; Amparo, Tatiane Roquete; Teixeira, Luiz Fernando de Medeiros; Vieira, Paula Melo de Abreu; Perasoli, Fernanda Barçante; Santos, Viviane Martins Rebello dosSynthetic polymers have been extensively studied in several areas of knowledge, one of which is the pharmaceutical. The drug when encapsultated into the PMMA-g-PEG 4000 matrix can produce fewer side effects. The most frequent side effects of erythromycin are gastrointestinal and are dose related. Nausea, vomiting and diarrhea occur in low frequency with the usual oral doses. The drug used in this study was Erythromycin, an antimicrobial that acts directly on bacterial protein synthesis. Erythromycin was encapsulated into PMMA-g-PEG 4000 derivatives. All copolymers afther encapsultaions were characterized and identified by atomic force microscopy (AFM). In vitro assays of release, antimicrobial activity and cytotoxicity were performed for the copolymers obtained. The copolymers PMMA-g-PEG 4000 HAL and PMMA-g-PEG 4000 ACET showed drug controlled release profiles. All PMMA-g-PEG 4000 derivatives showed antibacterial activity against Staphylococcus aureus and did not show a cytotoxic effect on human fibroblasts.Item In vitro and in silico investigation of the photoprotective and antioxidant potential of Protium spruceanum leaves and its main flavonoids.(2022) Amparo, Tatiane Roquete; Silva, Anne Cherem Peixoto da; Seibert, Janaína Brandão; Silva, Débora dos Santos da; Santos, Viviane Martins Rebello dos; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Souza, Gustavo Henrique Bianco de; Santos, Bianca Aloise Maneira CorrêaFor the first time, the photoprotective potential (UV absorption and antioxidant properties) of Protium spru- ceanum leaves and its main flavonoids was investigated combining an in vitro and in silico approach. The extract was standardized in rutin content (0.8% w/w), quantified by a validated HPLC-DAD method. The extract pro- tected fibroblasts and keratinocytes against cell death in the stress oxidative H2O2-induced test, with greater effect than rutin standard. This cytoprotection is correlated to the free radical scavenging action, determined by DPPH method. The theoretical investigation on the UV absorption showed an excellent correlation of the results (R2 = 0.911) allowing valuable information about the photoprotective properties of rutin, afzelin and quercitrin, as the importance of the A, B and C rings in the UV absorption property and the hydroxyls in B ring (catechol group) leading to a better antioxidant action. Therefore, the extract from P. spruceanum leaves is a promising candidate for sunscreen development.Item Reuse of hot trub as an active ingredient with antioxidant and antimicrobial potential.(2020) Costa, Fernanda Senna Ferreira; Amparo, Tatiane Roquete; Seibert, Janaína Brandão; Silveira, Benila Maria; Silva, Regislainy Gomes da; Pereira, Deise Inocêncio; Barbosa, Ramon Gontijo Garcia; Santos, Orlando David Henrique dos; Brandão, Geraldo Célio; Teixeira, Luiz Fernando de Medeiros; Vieira, Paula Melo de Abreu; Souza, Gustavo Henrique Bianco deThe reuse of agro-industrial residues is vital to minimize their environmental impact and alternatives for their application are needed. In this research, we evaluated the chemical composition as well as antioxidant and antibacterial activities of the extract from hot trub, that is the second solid waste obtained during the brewing process. Total phenolic and favonoids compounds were determined by colorimetric assays and the compounds were identifed by UHPLC-DAD-MS/MS. Antioxidant activity was evaluated by ABTS and DPPH methods. Antibacterial activity against Gram-positive and Gram-negative bacteria was analyzed by agar difusion and microdilution methods. Cytotoxicity was evaluated against murine fbroblast L929 cell line by MTT assay. The results showed that the content of phenolic and favonoid compounds ranged according to the fractions from 8.237 to 13.390 mg GAE/g and from 0.019 to 1.643 mg QE/g, respectively. The compounds cohumulone, cohulupone, hulupone/adhulupone, isoxanthohumol, xanthohumol, hydroxytricyclocolupone epimers, hydroxytricyclolupone/ hydroxytricicloadlupone epimers and nortricyclocolupone were suggested. Higher antioxidant activity was observed for the crude ethanol extract. The hexane fraction presented the lowest MIC value against Gram-positive bacteria, as well as lower cytotoxicity. These results are promising and indicate a great potential for the reuse of this byproduct as a source of natural antioxidant and for the microbiological applications, which consequently would reduce the environmental impact.