Navegando por Autor "Trajano, Eduardo Tavares Lima"

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Organ-related cigarette smoke-induced oxidative stress is strain-dependent.
(2010) Barroso, Carlos Romualdo Rueff; Trajano, Eduardo Tavares Lima; Alves, Jackson Nogueira; Paiva, Rojane Oliveira; Lanzetti, Manuella; Pires, Karla Maria Pereira; Bezerra, Frank Silva; Pinho, Ricardo Aurino; Valenca, Samuel Santos; Porto, Luís Cristovão de Moraes Sobrino
Background: Cigarette smoke (CS) is associated with oxidative stress in several organs because it contains high concentrations of free radicals and reactive oxygen species. Experimental models, using different strains, provide important insights into the genetic basis of diseases. This study sought to identify, in different mouse strains, the organ that is most-susceptible to CS-induced oxidative stress to obtain an optimized experimental animal model of oxidative injury induced by CS. Material/Methods: Male Swiss, DBA/2, C3H, BALB/c, and C57BL/6 mice were exposed to CS 3 times a day (4 cigarettes per session) for 60 consecutive days. Control groups from the same strains were sham-treated. Protein content, malondialdehyde level, myeloperoxidase activity, and nitrite level were assayed in lung, liver, kidney, and brain from all strains. Catalase and glutathione peroxidase activities were measured. Analyses of data were done by using a 1-way ANOVA with Bonferroni’s post-test (P<.05). Results: Cigarette smoke exposure resulted in distinct, organ-specific responses among strains. The survival rate of DBA/2 mice was lowest. BALB/c and C57BL/6 strains were more-susceptible to oxidative damage in the lung and liver. C3H and C57BL/6 mice were more-susceptible to oxidative damage in the brain. No renal oxidative damage was seen. Conclusions: Mouse strains and individual organs display a range of susceptibilities to CS-induced oxidative stress. BALB/c and C57BL/6 strains appear to be the best choices as experimental models for studying CS effects on liver and lung, and C3H and C57BL/6 strains for CS-effects on the brain.
Quantitative and morphological analyses of different types of human liver.
(2011) Nagato, Akinori Cardozo; Silva, Marco Aurélio dos Santos; Trajano, Eduardo Tavares Lima; Alves, Jackson Nogueira; Bandeira, Ana Carla Balthar; Ferreira, Tereza Aparecida; Valença, Samuel dos Santos; Bezerra, Frank Silva
Morphological variations in the human liver have been classified as congenital or acquired, although some may result from pseudo-injuries incurred during medical investigation. The present study comprises a systematic analysis of the anatomical variations exhibited by 61 formalinised and glycerinated adult human livers derived from a collection maintained at the Institute of Anatomy, Universidade Severino Sombra, Vassouras, RJ, Brazil. The vast majority of the organs analysed could be classified according to the seven morphological liver types previously established, although two additional liver types were identified and described. Detailed knowledge of anatomical variations in the human liver could be valuable in improving diagnostic procedures and in attaining a better understanding of pathological conditions associated with some liver diseases
The oxidative response of mouse hearts is modulated by genetic background.
(2013) Silva, Marco Aurélio dos Santos; Nagato, Akinori Cardozo; Trajano, Eduardo Tavares Lima; Alves, Jackson Nogueira; Bandeira, Ana Carla Balthar; Porto, Luís Cristovão de Moraes Sobrino; Bezerra, Frank Silva
Background: Smoking plays an important role in cardiovascular diseases. However, the reasons why some individuals develop those diseases and others do not remain to be explained. Objective: This study aimed at assessing the redox profile of the heart of different mouse strains after exposure to cigarette smoke. Methods: Male mice of the Swiss (n = 10), C3H (n = 10), BALB/c (n = 10) and C57BL/6 (n = 10) strains were exposed to cigarette smoke (12 cigarettes/day), while their respective controls (n = 10) were exposed to ambient air for 60 days. After being euthanized, their heart was removed for biochemical analyses. Results: Although the malondialdehyde content did not increase in any of the groups, catalase activity decreased in the Swiss (p < 0.05) and BALB/c (p < 0.05) strain mice as compared with their respective control groups, while myeloperoxidase decreased in the C3H (p < 0.05) and C57BL/6 (p < 0.001) strain mice as compared with their respective control groups. The reduced glutathione content decreased in the Swiss, C3H, C57BL/6 (p < 0.05) and BALB/c (p < 0,001) strain mice as compared with their respective control groups. Regarding reduced glutathione content, an increase was observed in the Swiss strain mice (p < 0.05), while a decrease was observed in the C3H (p < 0.05) and BALB/c (p < 0.001) strain mice as compared with their respective control groups. The reduced glutathione/reduced glutathione ratio showed a reduction in the Swiss and C57BL/6 (p < 0.05) strain mice as compared with their respective control groups. Conclusion: The genetic background of mice can influence the antioxidant response after exposure to cigarette smoke and seems to be a determinant factor for redox imbalance in Swiss and C57BL/6 strain mice. Understanding antioxidant responses and genetic background of C3H and BALB/c strain mice might provide important information regarding cardiac resistance to cigarette smoke.