Navegando por Autor "Reis, Thayane Oliveira"
Agora exibindo 1 - 6 de 6
Resultados por página
Opções de Ordenação
Item Abdominal TRPV1 channel desensitization enhances stress-induced hyperthermia during social stress in rats.(2023) Reis, Thayane Oliveira; Noronha, Sylvana Izaura Salyba Rendeiro de; Lima, Paulo Marcelo de Andrade; Abreu, Aline Rezende Ribeiro de; Mesquita, Laura Batista Tavares; Ferreira, Fernanda Isabel; Silva, Fernanda Cacilda dos Santos; Chianca Júnior, Deoclécio Alves; Menezes, Rodrigo Cunha Alvim deAims: In rats, stress-induced hyperthermia caused by social interaction depends on brown adipose tissue (BAT) thermogenesis and peripheral vasoconstriction. However, the peripheral mechanisms responsible for regulating the level of hyperthermia during social stress are still unknown. The transient receptor potential vanilloid 1 (TRPV1) subfamily, expressed in sensory and visceral neurons, can serve as a thermoreceptor. Here, we tested the hypothesis that the abdominal TRPV1 is essential in regulating stress-induced hyperthermia during social stress. Main methods: Male Wistar rats received an intraperitoneal injection of Resiniferatoxin (RTX) - an ultra-potent capsaicin analog, (i.e., to desensitize the TRPV1 channels) or vehicle. Seven days later, we evaluated the effects of abdominal TRPV1 channels desensitization on core body temperature (CBT), brown adipose tissue (BAT) temperature, tail skin temperature, and heart rate (HR) of rats subjected to a social stress protocol. Key findings: We found abdominal TRPV1 desensitization increased CBT and BAT temperature but did not change tail skin temperature and HR during rest. However, under social stress, we found that abdominal TRPV1 desensitization heightened the increase in CBT and BAT caused by stress. Also, it abolished the increase in tail skin temperature that occurs during and after social stress. TRPV1 desensitization also delayed the HR recovery after the exposure to the social stress. Significance: These results show that abdominal TRPV1 channels desensitization heightens stress-induced hyperthermia, causing heat dissipation during and after social stress, enabling optimal thermal control during social encounters.Item Lasting effects of ketamine and isoflurane administration on anxiety- and panic-like behavioral responses in Wistar rats.(2021) Chírico, Máira Tereza Talma; Guedes, Mariana Reis; Vieira, Lucas Gabriel; Reis, Thayane Oliveira; Santos, Aline Maria de; Souza, Ana Beatriz Farias de; Ribeiro, Iara Mariana Léllis; Noronha, Sylvana Izaura Salyba Rendeiro de; Nogueira, Katiane de Oliveira Pinto Coelho; Oliveira, Laser Antônio Machado de; Gomes, Fabiana Aparecida Rodrigues; Silva, Fernanda Cacilda dos Santos; Chianca Júnior, Deoclécio Alves; Bezerra, Frank Silva; Menezes, Rodrigo Cunha Alvim deIn clinical and laboratory practice, the use of anesthetics is essential in order to perform surgeries. Anesthetics, besides causing sedation and muscle relaxation, promote several physiological outcomes, such as psychotomimetic alterations, increased heart rate, and blood pressure. However, studies depicting the behavioral effect induced by ketamine and isoflurane are conflicting. In the present study, we assessed the behavioral effects precipitated by ketamine and isoflurane administration. We have also evaluated the ketamine effect on cell cytotoxicity and viability in an amygdalar neuronal primary cell culture. Ketamine (80 mg/kg) caused an anxiogenic effect in rats exposed to the elevated T-maze test (ETM) 2 and 7 days after ketamine administration. Ketamine (40 and 80 mg/kg) administration also decreased panic-like behavior in the ETM. In the light/dark test, ketamine had an anxiogenic effect. Isoflurane did not change animal behavior on the ETM. Neither ketamine nor isoflurane changed the spontaneous locomotor activity in the open field test. However, isoflurane-treated animals explored less frequently the OF central area seven days after treatment. Neither anesthetic caused oxidative damage in the liver. Ketamine also reduced cellular metabolism and led to neuronal death in amygdalar primary cell cultures. Thus, our work provides evidence that ketamine and isoflurane induce pronounced long lasting anxiety-related behaviors in male rats.Item Participação do receptor de potencial transitório vanilóide do tipo 1 (TRPV1) nas respostas termorregulatórias em ratos Wistar submetidos a estresse social.(2018) Reis, Thayane Oliveira; Menezes, Rodrigo Cunha Alvim de; Chianca Júnior, Deoclécio Alves; Menezes, Rodrigo Cunha Alvim de; Costa, Daniela Caldeira; Wanner, Samuel PennaA palavra estresse refere-se ao esforço da adaptação do organismo diante situações que são consideradas ameaçadoras à vida e ao equilíbrio interno. As respostas que o sistema nervoso central dos mamíferos produz durante o estresse envolvem aumento da concentração plasmática do hormônio adrenocorticotropico (ACTH), da pressão arterial (PA), da frequência cardíaca (FC), da temperatura do tecido adiposo marrom (TAM) e da temperatura corporal (TCO). Em ratos, as respostas fisiológicas para as situações marcantes incluem um aumento da temperatura corporal, que pode ser chamado de “hipertermia induzida por estresse”, que é mediada por uma combinação de termogênese do TAM e a vasoconstrição da artéria caudal. A temperatura dos tecidos periféricos é regulada e sincronizada por vias indiretas, pelos canais TRPV1, os quais atuam como mediadores na regulação da temperatura. O presente estudo é focado no canal TRPV1, um importante receptor de canal iônico pertencente à família TRP. Deste modo, objetivamos avaliar o papel do receptor TRPV1 periférico nas respostas cardíacas e termorregulatórias em situações de estresse social por rato intruso. Para tal, foram implantados nos animais sensores de FC e de temperatura e, após 24 horas, foi feito nos mesmos o protocolo de dessensibilização do TRPV1 com RTX (resiniferatoxina). As respostas cardíacas e termorregulatórias foram avaliadas após 7 dias durante o protocolo de indução de estresse social por rato intruso. Nossos resultados demonstraram que o estresse causou taquicardia, aumento na temperatura corporal e na temperatura do TAM nos animais veículo e dessensibilizados. Porém, após o período de estresse, a FC e as temperaturas corporal e do TAM não retornaram aos valores basais nos animais dessensibilizados. Observamos também que a dessensibilização do TRPV1, por si só, foi capaz de aumentar a temperatura do TAM e do dorso durante condições de repouso. Além disso, demonstramos que a dessensibilização dos canais TRPV1 diminuiu a temperatura caudal durante o período de estresse, ao contrário do grupo veículo, o qual aumentou a temperatura caudal durante estresse. Estes resultados mostraram que os animais dessensibilizados para TRPV1 com RTX, não recuperam seus valores basais de FC e temperatura após período de estresse. Assim, nosso estudo sugere que o TRPV1 é um importante receptor para a recuperação da temperatura após um período de estresse.Item The role of peripheral transient receptor potential vanilloid 1 channels in stress-induced hyperthermia in rats subjected to an anxiogenic environment.(2022) Lima, Paulo Marcelo de Andrade; Reis, Thayane Oliveira; Wanner, Samuel Penna; Chianca Júnior, Deoclécio Alves; Menezes, Rodrigo Cunha Alvim deAnxiety resulting from psychogenic stimuli elicit stress-induced hyperthermia in rats, often called “psychogenic fever”, which is part of a coordinated response to situations seen as novel or distressing. Brain transient receptor potential vanilloid 1 (TRPV1) channels modulate both thermoregulation and animal behavior; however, the role of peripheral TRPV1 channels in regulating these responses during exposure to an anxiogenic environment has not been determined. Thus, the present study aimed to investigate the involvement of abdominal TRPV1 channels in stress-induced hyperthermia and behavior in rats subjected to an unconditioned anxiety test. Desensitized rats (peripheral desensitization of TRPV1 channels with resiniferatoxin; RTX) and their respective controls were subjected to a 15-min open field (OF) test. The core body temperature (Tcore), tail skin temperature (Tskin), and rats’ movements inside the arena were recorded. The OF test induced a similar increase in Tcore in both groups throughout the exposure time; however, at the recovery period, the RTX-treated rats had a slower reduction in Tcore due to lower tail skin heat loss. Tskin decreased significantly in both groups during exposure to OF but, during recovery, the RTX-treated rats showed impaired skin vasodilation. Also, RTX-treated rats entered fewer times and spent less time in the OF center square, suggesting an anxiety-related behavior. Our findings indicate that, under stressful conditions, peripheral TRPV1 channels modulate thermoregulatory and behavioral responses. The TRPV1 desensitization induces a more prolonged hyperthermic response due to lower cutaneous heat dissipation, alongside a more evident anxiety-like behavior in rats subjected to the OF apparatus.Item Thermoregulatory responses, heart rate, and the susceptibility to anxiety in obese animals subjected to stress.(2023) Santos, Áquila Rodrigues Costa; Abreu, Aline Rezende Ribeiro de; Noronha, Sylvana Izaura Salyba Rendeiro de; Reis, Thayane Oliveira; Santos, Daisy Motta; Chianca Júnior, Deoclécio Alves; Silva Junior, Luiz Gonzaga da; Menezes, Rodrigo Cunha Alvim de; Rodrigues, Cibele VellosoObesity and stress are related to cardiovascular diseases. Rats fed a high-fat diet (HFD) show increased cardiovascular reactivity to emotional stress and altered defensive behavioral responses. Indeed, changes in thermoregulatory responses in an aversive environment are observed in these animals. However, studies aimed at clarifying the physiological mechanisms linking obesity, stress hyperreactivity and behavioral changes are needed. The aim of this study was to evaluate the changes in thermoregulatory responses, heart rate, and the susceptibility to anxiety in obese animals subjected to stress. Nine-week high-fat diet protocol was effective in inducing obesity by increasing weight gain, fat mass, adiposity index, white epididymal, retroperitoneal, inguinal and brown adipose tissue. Animals induced to obesity and subjected to stress (HFDS group) by the intruder animal method showed increases in heart rate (HR), core body temperature and tail temperature. HFDS showed an increase in the first exposure to the closed arm (anxiety-like behavior) in elevated T-Maze (ETM). The groups did not differ with respect to panic behavior assessed in the ETM and locomotor activity in the open field test. Our study shows that HFDS animals presented increased reactivity to stress with higher stress hyperthermia and anxious behavior. Thus, our results present relevant information regarding stress responsiveness and behavioral changes in obese animals.Item Tobacco-free cigarette smoke exposure induces anxiety and panic-related behaviours in male wistar rats.(2018) Chírico, Máira Tereza Talma; Bezerra, Frank Silva; Guedes, Mariana Reis; Souza, Ana Beatriz Farias de; Silva, Fernanda Cacilda dos Santos; Campos, Glenda Siqueira Viggiano; Noronha, Sylvana Izaura Salyba Rendeiro de; Mesquita, Laura Batista Tavares; Reis, Thayane Oliveira; Cangussú, Silvia Dantas; Chianca Júnior, Deoclécio Alves; Menezes, Rodrigo Cunha Alvim deSmokers, who generally present with lung damage, are more anxious than non-smokers and have an associated augmented risk of panic. Considering that lung damage signals specific neural pathways that are related to affective responses, the aim of the present study was to evaluate the influence of pulmonary injury on anxiety and panic-like behaviours in animals exposed to cigarette smoke with and without tobacco. Male Wistar rats were divided into the following groups: a control group (CG); a regular cigarette group (RC); and a tobacco-free cigarette (TFC) group. Animals were exposed to twelve cigarettes per day for eight consecutive days. The animals were then exposed to an elevated T-maze and an open field. The RC and TFC groups presented increases in inflammatory cell inflow, antioxidant enzyme activity, and TBARS levels, and a decrease in the GSH/GSSG ratio was observed in the TFC group. Exposure to RC smoke reduced anxiety and panic-related behaviours. On the other hand, TFC induced anxiety and panic-related behaviours. Thus, our results contradict the concept that nicotine is solely accountable for shifted behavioural patterns caused by smoking, in that exposure to TFC smoke causes anxiety and panic-related behaviours.