Navegando por Autor "Ferraz Filha, Zilma Schimith"

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Avaliação das atividades biológicas de espécies do gênero Lychnophora (arnicas) e estudo fitoquímico do extrato ativo de Lychnophora staavioides Mart.
(Programa de Pós-Graduação em Ciências Farmacêuticas. CIPHARMA, Escola de Farmácia, Universidade Federal de Ouro Preto., 2008) Ferraz Filha, Zilma Schimith; Guimarães, Dênia Antunes Saúde
Vinte e dois extratos de cinco espécies de Lychnophora e uma espécie de Lychnophoriopsis, tradicionalmente usadas no Brasil como analgésica, antiinflamatória e para o tratamento de reumatismo foram examinados para as atividades de inibição da xantina oxidase (XO), antioxidante e citotóxica. Realizou-se também o estudo fitoquímico do extrato clorofórmico de Lycnhophora staavioides (LS2) que apresentou atividade frente a todos os testes. Para a inibição da XO, enzima que catalisa o metabolismo de hipoxantina a xantina e esta a ácido úrico, foram testados dezesseis extratos. Para todos eles foi encontrada excelente atividade inibitória da XO, com inibição maior que 38% na concentração de 100 μg/mL na mistura ensaiada. As espécies com maior atividade foram Lychnophora ericoides, Lychnophora trichocarpha, Lychnophora staavioides, Lychnophoriopsis candelabrum, com inibição de 78%, 77%, 67% e 66% a 100 μg/mL, respectivamente, e valores de IC50 de 8,28; 6,16; 51,07 e 49,34 μg/mL, respectivamente. Para a determinação da atividade antioxidante, utilizou-se o radical livre DPPH (2,2-difenil-1-picril-hidrazila). A formação de radicais livres ocorre naturalmente no organismo devido à exposição ao oxigênio molecular. Eles são moléculas instáveis que contém um elétron desemparelhado levando a alterações e danos moleculares às células. Observou-se atividade antioxidante maior que 25% em doze dos vinte e dois extratos testados, na concentração de 100 μg/mL. As espécies com maior atividade foram Lychnophora passerina, Lychnophora trichocarpha, Lychnophora ericoides e Lychnophora pinaster, com inibição de 76,0%, 63,0%, 63,0% e 55,0% a 100 μg/mL, respectivamente. A atividade citotóxica foi avaliada através do teste larvicida com o microcrustáceo Artemia salina buscando a presença de lactonas sesquiterpênicas, marcadores químicos do gênero Lycnhophora¸ que possuem atividade antitumoral e citotóxica descrita. Vinte dois extratos foram testados. Observou-se que os extratos etanólicos brutos de L. trichocarpha, L. passerina, L. pinaster, L. ericoides e Lychnophoriopsis candelabrum, as frações hexânicas de L. candelabrum e L. ericoides, as frações clorofórmicas de L. candelabrum e L. staavioides, as frações acetato de etila de L. pinaster, L. trichocarpha, L. ericoides e a fração etanólica de L. candelabrum x apresentaram uma atividade citotˆxica menor que 1000 ‘g/mL e portanto podem conter as lactonas sesquiterpŠnicas. O estudo fitoqu‹mico do extrato clorofˆrmico de Lychnophora staavioides foi realizado visto que na triagem pelas atividades biolˆgicas esta esp‰cie mostrou-se ser promissora para a busca de subst ncias ativas. As partes a‰reas da planta foram extra‹das exaustivamente com hexano, clorofˆrmio e etanol. A fra†‡o clorofˆrmica foi submetida a novos fracionamentos, da qual foram isolados: uma mistura de triterpenos pentac‹clicos identificada como lupeol, α- e -amirinas, al‰m dos flavonˆides: 5-hidroxi- 7-metoxiflavona (tectocrisina), 5-hidroxi-7-metoxiflavanona (pinostrobina), 5-hidroxi-7- metoxiflavonol (isalpina) e 3β-O-acetoxi-5,7-diidroxi-2,3-diidroflavonol (pinobanksina - 3-O-acetato). Os quatro flavonˆides foram testados quanto ” atividade de inibi†‡o da XO, obtendo-se maior atividade para a subst ncia 3β-O-acetoxi-5,7-diidroxi-2,3- diidroflavonol na concentra†‡o de 100 ‘g/mL. As demais atividades n‡o foram avaliadas por n‡o haver quantidades suficientes das subst ncias puras.
Brine shrimp (Artemia salina Leach) bioassay of extracts from Lychnophoriopsis candelabrum and different Lychnophora species.
(2012) Ferraz Filha, Zilma Schimith; Lombardi, Júlio Antônio; Guzzo, Luciana Souza; Guimarães, Dênia Antunes Saúde
O presente estudo teve como objetivo avaliar a citotoxicidade sobre Artemia salina de vinte e dois extratos de cinco espécies do gênero Lychnophora e de uma espécie de Lychnophoriopsis. Os extratos solubilizados em DMSO, preparados nas concentrações finais de 100, 250, 375, 500 e 600 g mL-1, foram adicionados a recipientes contendo náuplios de Artemia salina (10 unidades cada) e completou-se o volume para 5 mL com solução marinha. Lapachol e DMSO 5% foram usados como controles positivo e negativo, respectivamente. As amostras foram mantidas sob iluminação e as larvas mortas foram contadas após 24 horas de contato. O cálculo da LC50 foi feito utilizando-se o programa Probitos. Os extratos etanólicos brutos de cinco espécies apresentaram baixa letalidade na seguinte ordem: Lychnophora trichocarpha (LC50 = 672,38 g mL-1) > Lychnophora pinaster (LC50 = 678,73 g mL-1) > Lychnophora ericoides (LC50 = 738,09 g mL-1) > Lychnophoriopsis candelabrum (LC50 = 812,57 g mL-1) > Lychnophora passerina (LC50 = 921,78 g mL-1). Todos os extratos testados de Lychnophoriopsis candelabrum e o extrato clorofórmico de Lychnophora staavioides mostraram leve toxicidade sobre A. salina. Os resultados indicaram que existem substâncias com potencial atividade farmacológica em todas as espécies testadas.
Effects of Pimenta pseudocaryophyllus extracts on gout : anti-inflammatory activity and anti-hyperuricemic effect through xantine oxidase and uricosuric action.
(2016) Ferrari, Fernanda Cristina; Lima, Rita de Cássia Lemos; Ferraz Filha, Zilma Schimith; Barros, Camila Helena; Araújo, Marcela Carolina de Paula Michel; Guimarães, Dênia Antunes Saúde
Ethnopharmacological relevance: Leave sin fusion of Pimenta pseudocaryophyllus (Gomes) Landrum is used in Brazilian folk medicine to treat the predisposition to arthritical and gouty affections of the joints, fever and other diseases. A refreshing drink prepared with the specie is al soused due to its diuretic, sedative and aphrodisiac actions. Aim of the study: The study was under taken to investigate the mechanisms of anti-hyperuricemic effect and anti-inflammatory activity of P. pseudocaryophyllus extracts. Materials and methods: Anti-hyperuricemic effect was investigated using xanthine oxidase assay and uricosuric studies with rats in which hyperuricemia was induced by potassium oxonate and uric acid. Anti-inflammatory activity was investigate don MSU crystal-induced paw edema model. Ethyl acetate extracts of the leaves (EAL) and branches (EAB), ethanolic extracts of leaves (EEL) and branches (EEB) and aqueous extracts of leaves (AL) and branches (AB) were evaluated. Results: The extracts of P. pseudocaryophyllus evaluated showed expressive results regarding the in-hibition of xanthine oxidase enzyme in vitro and they were also able to reduce serum uric acid levels in hyperuricemicrats. The investigation of the mechanism of action, it was found that EAL, EAB, EEB, AB (125and250mg/kg) and AL (250mg/kg) promoted an increase on the urinary excretion of uric acid and EEL, EEB, AB (125and250mg/kg) and EAB (250mg/kg) were capable to inhibit liver xanthine oxidase. Treatments with EEL (125and250mg/kg) and EEB (250mg/kg) were able to reduce edema at 48thh. EAL and EAB (125and250mg/kg) showed significant anti-inflammatory activity on monosodium urate crystal-induced paw edema model at all evaluated times. Conclusions: The specie P. pseudocaryophyllus showed remark able anti-hyperuricemic effects through uricosuric effects and inhibition of xanthine oxidase and therefore can be considered as a promise in the treatment of diseases related to hyperuricemia. Moreover, ethyl acetate extracts had significant anti- inflammatoryactivity.
Effects of the aqueous extract from Tabebuia roseoalba and phenolic acids on hyperuricemia and inflammation.
(2017) Ferraz Filha, Zilma Schimith; Ferrari, Fernanda Cristina; Araújo, Marcela Carolina de Paula Michel; Bernardes, Ana Catharina Fernandes Pereira Ferreira; Guimarães, Dênia Antunes Saúde
Tabebuia species (Bignoniaceae) have long been used in folk medicine as anti-inflammatory, antirheumatic, antimicrobial, and antitumor. The aim of this study was to investigate if aqueous extract from the leaves (AEL) of Tabebuia roseoalba (Ridl.) Sandwith, Bignoniaceae, and its constituents could be useful to decrease serum uric acid levels and restrain the gout inflammatory process. HPLC analysis identified caffeic acid and chlorogenic acid in AEL. Antihyperuricemic effects and inhibition of liver XOD (xanthine oxidoreductase) by AEL and identified compounds were evaluated in hyperuricemic mice. Anti-inflammatory activity was evaluated on MSU (monosodium urate) crystal-induced paw edema. In addition, AEL antioxidant activity in vitro was evaluated. AEL, caffeic, and chlorogenic acids were able to reduce serum uric acid levels in hyperuricemic mice probably through inhibition of liver xanthine oxidase activity and significantly decreased the paw edema induced by MSU crystals. AEL showed significant antioxidant activity in all evaluated assays. The results show that the AEL of Tabebuia roseoalba can be a promising agent for treatment for gout and inflammatory diseases. We suggest that caffeic and chlorogenic acids may be responsible for the activities demonstrated by the species.
The influence of seasonality on the content of goyazensolide and on anti-inflammatory and anti-hyperuricemic effects of the ethanolic extract of Lychnophora passerina (Brazilian arnica).
(2017) Ugoline, Bruno César de Albuquerque; Souza, Jacqueline de; Ferrari, Fernanda Cristina; Ferraz Filha, Zilma Schimith; Coelho, Grazielle Brandão; Guimarães, Dênia Antunes Saúde
Ethnopharmacological relevance: Lychnophora passerina (Mart ex DC) Gardn (Asteraceae), popularly known as Brazilian arnica, is used in Brazilian folk medicine to treat pain, rheumatism, bruises, inflammatory diseases and insect bites. Aim of the study: Investigate the influence of the seasons on the anti-inflammatory and anti-hyperuricemic activities of ethanolic extract of L. passerina and the ratio of the goyazensolide content, main chemical constituent of the ethanolic extract, with these activities. Materials and methods: Ethanolic extracts of aerial parts of L. passerina were obtained from seasons: summer (ES), autumn (EA), winter (EW) and spring (EP). The sesquiterpene lactone goyazensolide, major metabolite, was quantified in ES, EA, EW and EP by a developed and validated HPLC-DAD method. The in vivo antihyperuricemic and anti-inflammatory effects of the ethanolic extracts from L. passerina and goyazensolide were assayed on experimental model of oxonate-induced hyperuricemia in mice, liver xanthine oxidase (XOD) inhibition and on carrageenan-induced paw edema in mice. Results: HPLC method using aqueous solution of acetic acid 0.01% (v/v) and acetonitrile with acetic acid 0.01% (v/v) as a mobile phase in a gradient system, with coumarin as an internal standard and DAD detection at 270 nm was developed. The validation parameters showed linearity in a range within 10.0–150.0 μg/ml, with intraday and interday precisions a range of 0.61–3.82. The accuracy values of intraday and interday analysis within 87.58– 100.95%. EA showed the highest goyazensolide content. From the third to the sixth hour after injection of carrageenan, treatments with all extracts at the dose of 125 mg/kg were able to reduce edema. Goyazensolide (10 mg/kg) showed significant reduction of paw swelling from the second hour assay. This sesquiterpene lactone was more active than extracts and presented similar effect to indomethacin. Treatments with ES, EA and EP (125 mg/kg) and goyazensolide (10 mg/kg) reduced serum urate levels compared to hyperuricemic control group and were able to inhibit liver XOD activity. One of the mechanisms by which ES, EA, EP and goyazensolide exercise their anti-hyperuricemic effect is by the inhibition of liver XOD activity. Goyazensolide was identified as the main compound present in ES, EA, EW and EP and it is shown to be one of the chemical constituents responsible for the anti-inflammatory and anti-hyperuricemic effects of the ethanolic extracts. Conclusion: The anti-inflammatory and anti-hyperuricemic activities of the ethanolic extracts from L. passerina were not proportionally influenced by the variation of goyazensolide content throughout the seasons. The involvement of goyazensolide on in vivo anti-inflammatory and anti-hyperuricemic activities of L. passerina extracts was confirmed, as well as the possibility of participation of other constituents on these effects. This study demonstrated that the aerial parts of L. passerina may be collected in any season for use as anti-inflammatory agent. For use in hyperuricemia, the best seasons for the collection are summer, autumn and spring. The ethanolic extract of L. passerina and goyazensolide can be considered promising agents in the therapeutic of inflammation, hyperuricemia and gout.