Navegando por Autor "Alberti, Luiz Ronaldo"
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Item Comparison of the efficacy of losartan, hydrocortisone and acetylsalicylic acid (ASA) in preventing the development of fibrous scar tissue in skeletal muscle.(2018) Silva Júnior, Otávio de Melo; Nunes, Cristiana Buzelin; Corrêa, Tchalton Amador; Silva, Marilia F.; Freitas, Laurice Barbosa; Alberti, Luiz RonaldoObjective: To analyze fibrous scar tissue inhibition capacity with the use of losartan, hydrocortisone and acetylsalicylic acid. Method: The sample consisted of 120 male heterogeneic Wistar rats with a muscle laceration model. The rats were divided into four groups of 30 animals each: control group, losartan group, ASA group and hydrocortisone group. The animals were anesthetized and a 2.5 cm longitudinal incision was made in the left thoracolumbar paravertebral region. The muscles were subjected to a Grade III lesion caused by applying Kelly hemostatic forceps for 60 seconds, followed by sectioning with scissors. The skin was sutured with 3-0 nylon monofilament thread. The animals were placed in individual cages with plenty of food and water. The losartan group received losartan diluted in water at a dose of 0.1 mg/mL (10 mg/kg/day), the ASA Group received a 3 mg/mL ASA solution (300 mg/kg/day), and the hydrocortisone group received a 0.2 mg/mL hydrocortisone solution (20 mg/kg/day). Results: The control, losartan, hydrocortisone and aspirin groups had a fibrotic area of 0.95 ± 0.35 mm, 0.55 ± 0.34 mm, 0.93 ± 0.33 mm, and 0.66 ± 0.36 mm, respectively. We observed a significantly smaller fibrotic area in the losartan group compared to the control (p=0.01) and hydrocortisone (p=0.01) groups. There were no significant differences among the other groups. Conclusion: The healing of striated skeletal muscle produced less fibrous scar tissue when exposed to losartan in comparison to the control group or the hydrocortisone group. Level of Evidence I; Randomized double-blind placebo-controlled study.Item Effects of clonidine, isoflurane, and the clonidine+isoflurane association in isolated hearts.(2023) Dupin, João Bosco; Alberti, Luiz Ronaldo; Zocratto, Orlando BarretoBackground: Isoflurane has been consecrated as an anesthetic drug of choise in heart surgeries, as it presents characteristics that ensure the preservation of cardiac indexes and myocardial stability. Recently, alpha-adrenergic agents, mainly Clonidine, have been added to this anesthetic arsenal in an attempt to prevent adrenergic discharges, increase cardiac stability, reduce myocardial ischemia and improve anesthetic induction and recovery. Objective: The aim of this study was to evaluate the cardiovascular effects of Clonidine, Isoflurane, and Clonidine+Isoflurane association in isolated hat hearts preparations, as well as to evaluate direct heart effects of the clonidine, possibly masked by its central action. Method: This study used twenty four, male, albino, Wistar rats from the Biotherium of the Federal University of Minas Gerais (UFMG). The animals were anesthetized with 100 mg of ketamine + 10 mg of xylazine intraperitoneally, and, after a full thoracotomy, their hearts were isolated and placed in coronary perfusion using a Krebs-Henseleit nutrient-rich solution, according to the Langerdorff method. The parameters of Heart Rate, Systolic Blood Pressure, Coronary Flow, and Myocardial Contractility were evaluated at times of O, 1, 2, 3, 5, 10, and 15 minutes. Results: Heart rate – No statistically significant difference was observed among the Clonidine, Isoflurane, Clonidine+Isoflurane, and Control groups. Systolic Blood Pressure – No statistically significant difference was identified among the Clonidine, Clonidine+Isoflurane, and Control groups. In the group that received only Isoflurane, the systolic blood pressure proved to be equal to the control group and presented, on average, 18.3 more units than did the Clonidine group. In the groups that received Clonidine+Isoflurane, the systolic blood pressure was, on average, 22.5 units less than thecontrol group and, on average, 32.7 units less than the group that received only Isoflurane. Coronary Flow – No statistically significant difference was found regarding the coronary flow among the Clonidine, Isoflurane, Clonidine+Isoflurane, and Control groups. Myocardial Contractility – No statistically significant change was found in this parameter among the Clonidine, Isoflurane, Clonidine+Isoflurane, and Control groups. Conclusion: Clonidine, when used in an isolated manner, produced no significant effect on the hemodynamic behavior of the isolated rat hearts. When used in association with Isoflurane, Clonidine was capable of diminishing the effects of this drug, demonstrating an apparent protective effect upon the heart. The observed effects occurred directly upon the heart, considering that this study was conducted on isolated hearts, that is, with no connection to the central nervous system.