Carvalho, Eduardo Butturini deRamos, Isalira Peroba RezendeNascimento, Alvaro Fernando da Silva doBrasil, Guilherme ViscondeMello, Debora BastosOti, MartinSammeth, MichaelBahia, Maria TerezinhaCarvalho, Antônio Carlos Campos deCarvalho, Adriana Bastos2020-04-242020-04-242019CARVALHO, E. B. et al. Echocardiographic measurements in a preclinical model of chronic chagasic cardiomyopathy in dogs: validation and reproducibility. Frontiers in Cellular and Infection Microbiology, v. 9, p. 332, set. 2019. Disponível em: <https://www.frontiersin.org/articles/10.3389/fcimb.2019.00332/full>. Acesso em: 10 fev. 2020.2235-2988http://www.repositorio.ufop.br/handle/123456789/12098Background: The failure to translate preclinical results to the clinical setting is the rule, not the exception. One reason that is frequently overlooked is whether the animal model reproduces distinctive features of human disease. Another is the reproducibility of the method used to measure treatment effects in preclinical studies. Left ventricular (LV) function improvement is the most common endpoint in preclinical cardiovascular disease studies, while echocardiography is the most frequently used method to evaluate LV function. In this work, we conducted a robust echocardiographic evaluation of LV size and function in dogs chronically infected by Trypanosoma cruzi. Methods and Results: Echocardiography was performed blindly by two distinct observers in mongrel dogs before and between 6 and 9 months post infection. Parameters analyzed included end-systolic volume (ESV), end-diastolic volume (EDV), ejection fraction (EF), and fractional shortening (FS). We observed a significant LVEF and FS reduction in infected animals compared to controls, with no significant variation in volumes. However, the effect of chronic infection in systolic function was quite variable, with EF ranging from 17 to 66%. Using the cut-off value of EF ≤ 40%, established for dilated cardiomyopathy (DCM) in dogs, only 28% of the infected dogs were affected by the chronic infection. Conclusions: The canine model of CCC mimics human disease, reproducing the percentage of individuals that develop heart failure during the chronic infection. It is thus mandatory to establish inclusion criteria in the experimental design of canine preclinical studies to account for the variable effect that chronic infection has on systolic function.en-USabertoChagas diseaseCanine modelSystolic dysfunctionArtigo publicado em periodicoThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Fonte: o próprio artigo.https://doi.org/10.3389/fcimb.2019.00332