Correa, Maria Adelaida DuqueGoulding, DavidRodgers, Faye H.Gillis, J. AndrewCormie, ClaireRawlinson, Kate A.Bancroft, Allison J.Bennett, Hayley M.Lotkowska, Magda E.Reid, Adam J.Speak, Anneliese O.Scott, PaulRedshaw, NicholasTolley, CharlotteMcCarthy, CatherineBrandt, CordeliaSharpe, CatherineRidley, CarolineMoya, Judit GaliCarneiro, Cláudia MartinsStarborg, TobiasHayes, Kelly S.Holroyd, NancySanders, MandyThornton, David J.Grencis, Richard K.Berriman, Matthew2023-10-112023-10-112022CORREA, M. A. D. et al. Defining the early stages of intestinal colonisation by whipworms. Nature Communications, v. 13, artigo 1725, 2022. Disponível em: <https://www.nature.com/articles/s41467-022-29334-0>. Acesso em: 01 ago. 2023.2041-1723http://www.repositorio.ufop.br/jspui/handle/123456789/17568Whipworms are large metazoan parasites that inhabit multi-intracellular epithelial tunnels in the large intestine of their hosts, causing chronic disease in humans and other mammals. How first-stage larvae invade host epithelia and establish infection remains unclear. Here we investigate early infection events using both Trichuris muris infections of mice and murine caecaloids, the first in-vitro system for whipworm infection and organoid model for live helminths. We show that larvae degrade mucus layers to access epithelial cells. In early syncytial tunnels, larvae are completely intracellular, woven through multiple live dividing cells. Using single-cell RNA sequencing of infected mouse caecum, we reveal that progression of infection results in cell damage and an expansion of enterocytes expressing of Isg15, potentially instigating the host immune response to the whipworm and tissue repair. Our results unravel intestinal epithelium invasion by whipworms and reveal specific host-parasite interactions that allow the whipworm to establish its multi-intracellular niche.pt-BRabertoArtigo publicado em periodicoThis article is under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. Fonte: PDF do artigo.https://doi.org/10.1038/s41467-022-29334-0