Souza, Juliana Vitoriano deMathias, Fernando Augusto SiqueiraMoreira, Nádia das DoresSoares, Rodrigo Dian de Oliveira AguiarVieira, Paula Melo de AbreuCarvalho, Andréa Teixeira deCarneiro, Cláudia MartinsGiunchetti, Rodolfo CordeiroBrito, Rory Cristiane Fortes deFujiwara, Ricardo ToshioRoatt, Bruno MendesMelo, Maria NormaReis, Alexandre Barbosa2020-05-082020-05-082019SOUZA, J. V. et al. Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and incomplete freund’s adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation. Vaccine, v. 37, p. 7269-7279, nov. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0264410X19312952?via%3Dihub>. Acesso em: 10 fev. 2020.2076-393Xhttp://www.repositorio.ufop.br/handle/123456789/12178The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response. Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response. In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund’s Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE ) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL. The specific immune response induced by these compositions demonstrated that they were powerfully immunogenic, increasing cellular infiltration in the skin. Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+ CD86+ cells. SAP, MPL, LBSAP, LBIFA and LBMPL could activate lymphocytes increasing expression of CD69 and CD25. LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h. At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups. Our data demonstrate that LBSAP and LBMPL are potential formulations to be tested in other experimental models. Also, the data obtained could expand the knowledge about immune response after sensitization and also contribute to the development of safe, immunogenic and effective vaccines.en-USrestritoCellular infiltrationCytokinesArtigo publicado em periodicohttps://www.sciencedirect.com/science/article/pii/S0264410X19312952?via%3Dihubhttps://doi.org/10.1016/j.vaccine.2019.09.067