Cunha, Aureliano Claret daGomes, Lorena SoaresSantos, Fernanda GodoyOliveira, Fábio FariaTeixeira, Janaina AparecidaSampaio, Geraldo Magela SantosTrópia, Maria José MagalhãesCastro, Ieso de MirandaLucas, Cândida Manuel Ribeiro SimõesBrandão, Rogélio Lopes2019-04-222019-04-222019CUNHA, A. C. da et al. High‑affinity transport, cyanide‑resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus. Journal of Industrial Microbiology & Biotechnology, v. 2019, p. 1-15, 2019. Disponível em: <https://link.springer.com/article/10.1007%2Fs10295-018-02119-5>. Acesso em: 7 mar. 2019.1476-5535http://www.repositorio.ufop.br/handle/123456789/11075Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.en-USrestritoGlycerol metabolismCachaçaSTL1Biotechnological applicationsArtigo publicado em periodicohttps://link.springer.com/article/10.1007%2Fs10295-018-02119-5