Ferreira, Andrea da FonsecaFrança, Flávia DayrellRossoni Júnior, Joamyr VictorMoraes, Karen Cristiane Martinez deGomes, Dawidson AssisCosta, Daniela CaldeiraTagliati, Carlos AlbertoChaves, Míriam Martins2017-07-042017-07-042015FERREIRA, A. da F. et al. Cyclic adenosine monophosphate protects renal cell lines against amphotericin B toxicity in a PKA-independent manner. Drug and Chemical Toxicology, New York, v. 07, p. 1-7, 2015. Disponível em: <http://www.tandfonline.com/doi/abs/10.3109/01480545.2015.1012210?journalCode=idct20>. Acesso em: 16 jun. 2017.1525-6014http://www.repositorio.ufop.br/handle/123456789/8140Amphotericin B is the ‘‘gold standard’’ agent in the management of serious systemic fungal infections. However, this drug can cause nephrotoxicity, which contributes up to 25% of all acute kidney injuries in critically ill patients. Cyclic adenosine monophosphate can protect kidney cells from death due to injury or drug exposure in some cases. Hence, the objective of this work was to evaluate if cAMP could prevent cell death that occurs in renal cell lines subjected to AmB treatment and, if so, to assess the involvement of PKA in the transduction of this signal. Two different renal cell lines (LLC-PK1 and MDCK) were used in this study. MTT and flow cytometry assays showed increased cell survival when cells were exposed to cAMP in a PKA-independent manner, which was confirmed by western blot. This finding suggests that cAMP (db-cAMP) may prevent cell death caused by exposure to AmB. This is the first time this effect has been identified when renal cells are exposed to AmB’s nephrotoxic potential.en-USrestritoCell protectionNephrotoxicityArtigo publicado em periodicohttp://www.tandfonline.com/doi/abs/10.3109/01480545.2015.1012210?journalCode=idct20https://doi.org/10.3109/01480545.2015.1012210