Papini, Tatiane Figueiredo MoraisReis, Jordana Grazziela Alves Coelho dosWendling, Ana Paula BarbosaAntonelli, Lis Ribeiro do ValleWowk, Pryscilla FaniniBonato, Vânia Luiza DeperonAugusto, Valéria MariaSantos, Silvana Maria ElóiMartins Filho, Olindo AssisCarneiro, Cláudia MartinsCarvalho, Andréa Teixeira de2017-08-302017-08-302017PAPINI, T. F. M. et al. Systemic Immunological changes in patients with distinct clinical outcomes during Mycobacterium tuberculosis infection. Immunobiology, v. 22, n. 11, p. 1014-1024, nov. 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0171298517300992?via%3Dihub>. Acesso em: 29 ago. 2017.0171-2985http://www.repositorio.ufop.br/handle/123456789/8585Background: The lung lesions in an individual infected with tuberculosis (TB) are surprisingly variable and independent of each other. However, there is no circulating biomarker yet able to segregate patients according to the extent of lung lesions. Materials and methods: In this study, the phenotypic and functional profile of leukocytes of patients with active pulmonary tuberculosis (TB) and controls (CO) were fully scrutinized by immunophenotyping assays and in vitro short-term whole blood culture. The TB group was subdivided according to the extent of lung lesions as unilateral (UNI) and bilateral (BI). Results: The results show that TB group display an altered leukocyte profile in the peripheral blood with significant lower counts of NK-cells, CD3+CD56+CD16+/− NKT-cells, CD4+T-cells, CD19+B-cells when compared to CO. Increased CD4+T-cells and CD8+T-cell activation was observed by the upregulation of activation markers (HLA-DR) as well as of chemokine receptors (CCR2, CCR3, and CXCR4). In addition, TB group presented a significant decrease proportion of CD14LowCD16+ monocytes despite the increase in HLA-DR expression. Regarding the severity of the disease, in the BI group a reduction in frequency of CD19+CD5+ B-cells and expression of HLA-DR in CD14LowCD16+ monocytes was observed. Furthermore, the extent of lung lesions influences the production of molecules as observed by significantly larger production of IL-4 by neutrophils, total T-cells, CD4+T-cells, CD8+T-cells and CD19+B-cells in UNI as compared to BI. By contrast, in BI group the frequency of high producers of both IL-17+CD4+T-cells and IL-17+CD8+T-cells were significantly increased than UNI, suggesting the deleterious role of these subsets during active pulmonary Mtb infection. Conclusion: The immunophenotypic characterization of unilateral and bilateral active TB performed in the present study indicates that the extent of lung lesion could be associated with a fine-tuning between immunological responses during untreated Mtb infection.en-USabertoImmune responseImmunophenotypingArtigo publicado em periodicoO periódico Immunobiology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 4178330595335.https://doi.org/10.1016/j.imbio.2017.05.016