Silva, Eduardo de Almeida Marques daCoelho, Eduardo Antônio FerrazGomes, Daniel Cláudio de OliveiraVilela, Márcia de CarvalhoMasioli, Cássio ZumerleTavares, Carlos Alberto PereiraFernandes, Ana Paula Salles MouraAfonso, Luís Carlos CroccoRezende, Simone Aparecida2017-04-072017-04-072005SILVA, E. A. M. da et al. Intramuscular immunization with p36(LACK) DNA vaccine induces IFN-gama production but does not protect BALB/c mice against Leishmania chagasi intravenous challenge. Parasitology Research, v. 98, n.1, p. 67-74, 2005. Disponível em: <http://link.springer.com/article/10.1007%2Fs00436-005-0008-8>. Acesso em: 20 jan. 2017.1432-1955http://www.repositorio.ufop.br/handle/123456789/7577Acute visceral leishmaniasis is a progressive disease caused by Leishmania chagasi in South America. The acquisition of immunity following infection suggests that vaccination is a feasible approach to protect against this disease. Since Leishmania homologue of receptors for activated C kinase (LACK) antigen is of particular interest as a vaccine candidate because of the prominent role it plays in the pathogenesis of experimental Leishmania major infection, we evaluated the potential of a p36(LACK) DNA vaccine in protecting BALB/c mice challenged with L. chagasi. In this study, mice received intramuscular (i.m.) or subcutaneous (s.c.) doses of LACK DNA vaccine. We evaluated the production of vaccine-induced cytokines and whether this immunization was able to reduce parasite load in liver and spleen. We detected a significant production of interferon gamma by splenocytes from i.m. vaccinated mice in response to L. chagasi antigen and to rLACK protein. However, we did not observe a reduction in parasite load neither in liver nor in the spleen of vaccinated animals. The lack of protection observed may be explained by a significant production of IL-10 induced by the vaccine.en-USrestritoLackArtigo publicado em periodicohttp://link.springer.com/article/10.1007%2Fs00436-005-0008-8https://doi.org/10.1007/s00436-005-0008-8