Miranda, Silvana Spindola deAlmeida, Isabela Neves deMansur, Maria de Fátima Filardi OliveiraFigueredo, Lida Jouca de AssisCarvalho, Wânia da SilvaHadaad, João Paulo AmaralDiniz, Jaciara de Lourdes do Carmo GuimarãesGroll, Andrea vonSilva, Pedro Almeida daLopes, Maria LuizaSantos, Marcelo Cordeiro dosBrito, AlexandraMello, Fernanda Carvalho de QueirozMalaquias, Thiago da Silva SantosCroda, JulioPinhata, Juliana Maira WatanabeOliveira, Rosângela Siqueira deChimara, EricaRossetti, Maria LúciaHalon, Maria LauraLourenço, Maria CristinaMedeiros, Reginalda Ferreira de MeloMontes, Fátima Cristina Onofre FandinhoMachado, DianaViveiros, MiguelKritski, Afrânio Lineu2023-01-162023-01-162020MIRANDA, S. S. de et al. Detection of drug resistant mycobacterium tuberculosis strains using Kit SIRE Nitratase®: a multicenter study. Brazilian Archives of Biology and Technology, v. 63, 2020. Disponível em: <https://www.scielo.br/j/babt/a/rwZ8F449jqmSRmgWcqgFdxc/?lang=en>. Acesso em: 11 out. 2022.1678-4324http://www.repositorio.ufop.br/jspui/handle/123456789/15951(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.en-USabertoMultidrug resistant tuberculosisNitrate reductaseTechnologyArtigo publicado em periodicoSubmitted for possible open access publication under the terms and conditions of the Creative Commons Attribution (CC BY NC) license (https://creativecommons.org/licenses/by-nc/4.0/). Fonte: o PDF do artigo.https://doi.org/10.1590/1678-4324-2020190179