Soyer, Tauane GonçalvesRamos, Fernanda FonsecaPereira, Isabela Amorim GonçalvesLage, Daniela PagliaraBandeira, Raquel SoaresJesus, Marcelo Moreira deCosta, Guilherme de PaulaMachado, Amanda SanchezFreitas, Camila Simões deVale, Danniele LucianaMartins, Vivian TamiettiGaldino, Alexsandro SobreiraChávez Fumagalli, Miguel AngelSouza, Daniel MenezesDuarte, Mariana CostaRoatt, Bruno MendesCoelho, Eduardo Antônio FerrazTavares, Grasiele de Sousa Vieira2023-10-312023-10-312023SOYER, T. G. et al. Immunotherapy using immunogenic mimotopes selected by phage display plus amphotericin B inducing a therapeutic response in mice infected with Leishmania amazonenses. Pathogens, v. 12, n. 2, p. 314-335, 2023. Disponível em: <https://www.mdpi.com/2076-0817/12/2/314>. Acesso em: 01 ago. 2023.2076-0817http://www.repositorio.ufop.br/jspui/handle/123456789/17701Leishmania amazonensis can cause cutaneous and visceral clinical manifestations of leish- maniasis in infected hosts. Once the treatment against disease is toxic, presents high cost, and/or there is the emergence of parasite-resistant strains, alternative means through which to control the disease must be developed. In this context, immunotherapeutics combining known drugs with immunogens could be applied to control infections and allow hosts to recover from the disease. In this study, immunotherapeutics protocols associating mimotopes selected by phage display and amphotericin B (AmpB) were evaluated in L. amazonensis-infected mice. Immunogens, A4 and A8 phages, were administered alone or associated with AmpB. Other animals received saline, AmpB, a wild-type phage (WTP), or WTP/AmpB as controls. Evaluations performed one and thirty days after the application of immunotherapeutics showed that the A4/AmpB and A8/AmpB combinations induced the most polarized Th1-type immune responses, which reflected in significant reductions in the lesion’s average diameter and in the parasite load in the infected tissue and distinct organs of the animals. In addition, the combination also reduced the drug toxicity, as compared to values found using it alone. In this context, preliminary data presented here suggest the potential to associate A4 and A8 phages with AmpB to be applied in future studies for treatment against leishmaniasis.en-USabertoImmunotherapeuticsTegumentary leishmaniasisImmune responseArtigo publicado em periodicoThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: PDF do artigo.https://doi.org/10.3390/pathogens12020314