Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.

Dutra, Alexandre Augusto de AssisSousa, Lucas OliveiraResende, Rodrigo RibeiroBrandão, Rogélio LopesKalapothakis, EvanguedesCastro, Ieso de Miranda2012-07-182012-07-182008DUTRA, A. A. A. et al. Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels. Peptides, v. 29, n. 9, p. 1505-1513, set. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0196978108002131. Acesso em: 18 jul. 2012.01969781http://www.repositorio.ufop.br/handle/123456789/1165Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.en-USCalcium channelVenomSpider toxinsLasiodoracDNAExpressionExpression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.Artigo publicado em periodicoO periódico Peptides concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3266011353103.