Queiroz, Pollyana SantosRuas, France Anne DiasBarboza, Natália RochaBorges, William de CastroCota, Renata Guerra de Sá2019-04-092019-04-092018QUEIROZ, P. S. et al. Alterations in the proteomic composition of Serratia marcescens in response to manganese (II). BMC Biotechnology, v. 18, n. 83, p. 1-9, 2018. Disponível em: <https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-018-0493-3>. Acesso em: 25 fev. 2019.14726750http://www.repositorio.ufop.br/handle/123456789/10984Background: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). Results: The LG1 strain, which grew equally well in the two conditions, was found to express a set of proteins related to cellular processes vital for survival, as well as proteins involved in adaptation and tolerance to Mn (II). The multicopper oxidase CueO was identified, indicating its probable participation in the Mn (II) bio-oxidation; however, its expression was not modulated by the presence of Mn (II). A set of proteins related to cell and metabolic processes vital to the cells were downregulated in the presence of Mn (II), while cell membrane-related proteins involved in the maintenance of cell integrity and survival under stress were upregulated under this condition. Conclusions: These findings indicate that the LG1 strain may be applied successfully in the bioremediation of Mn (II), and the shotgun approach provides an efficient means for obtaining the total proteome of this species.en-USabertoMulticopper oxidaseSerratia marcescens proteomeMn (II) oxidationMn (II)-modulated protein expressionArtigo publicado em periodicoThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Fonte: O próprio artigo.