Prado, Marco Antônio MaximoSantos, T. MoraesFreitas, Renata Nascimento deSilva, Marco Aurélio RomanoGomez, Marcus Vinicius2015-04-012015-04-011990PRADO, M. A. M. et al. Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices. Journal of Neuroscience Methods, v. 31, p. 193-196, 1990. Disponível em: <https://www.sciencedirect.com/science/article/pii/0165027090901102>. Acesso em: 08 nov. 2014.0165-0270http://www.repositorio.ufop.br/handle/123456789/4916A chemiluminescent method has been used recently for the determination of acetylcholine with limitations such as the presence of a cholinesterase inhibitor in the incubation medium, which is indispensable for the study of acetylcholine release by various agents. A modified procedure is presented in which the cholinesterase inhibitor eserine (physostigmine) is extracted from the medium. The results showed complete recovery when labelled acetylcholine was used. This modified procedure was used to determine the release of acetylcholine evoked by tityustoxin and ouabain. The results were comparable to those obtained by bioassay using a strip of guinea pig ileum.en-USAcetylcholine assayChemiluminescent procedureNeurotransmitter releaseArtigo publicado em periodicoO periódico Journal of Neuroscience Methods concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3556580424364.https://doi.org/10.1016/0165-0270(90)90110-2