Valentim, Cláudia Laignier LageGomes, Matheus de SouzaJeremias, Wander de JesusCunha, Juliana CecíliaOliveira, Guilherme Corrêa deBotelho, Ana Cristina de CarvalhoPimenta, Paulo Filemon PaolucciPassos, Liana Konovaloff JannottiCota, Renata Guerra de SáBabá, Élio Hideo2017-06-232017-06-232008VALENTIM, C. L. L. et al. Physical localization of the retrotransposons Boudicca and Perere 03 in Schistosoma mansoni. The Journal of Parasitology, v. 94, p. 993-994, 2008. Disponível em: <https://goo.gl/axLYEk>. Acesso em: 20 mar. 2017.1937-2345http://www.repositorio.ufop.br/handle/123456789/8057Schistosoma mansoni is 1 of the causative agents of schistosomiasis, an endemic disease in 76 countries of the world. The study of its genome, estimated to be 270 Mb, is very important to understanding schistosome biology, the mechanisms of drug resistance, and immune evasion. Repetitive elements constitute more than 40% of the S. mansoni genome and may play a role in the parasite evolution. The retrotransposons Boudicca, a long terminal repeat (LTR), and Perere 03, a non-LTR, are present in a high number in the S. mansoni genome and were localized with the use of fluorescence in situ hybridization (FISH) and primed in situ labeling (PRINS). Bacterial artificial chromosomes (BAC) clones containing the retrotransposons Boudicca and Perere 03 were selected by bioinformatic analysis and used as probes in FISH. Using metaphase chromosomes from sporocysts and the FISH and PRINS techniques, we were able to map these retrotransposons. Perere 03 was localized in the euchromatic regions of the short arm of chromosome 2 and Boudicca inen-USrestritoPhysical localization of the retrotransposons Boudicca and Perere 03 in Schistosoma mansoni.Artigo publicado em periodicohttps://goo.gl/axLYEkhttps://doi.org/10.1645/GE-1167.1