Siqueira, Raoni PaisMilagre, Matheus MarquesOliveira, Maria Alice deBranquinho, Renata TupinambáTorchelsen, Fernanda Karoline Vieira da SilvaLana, Marta deMachado, Marina Guimarães CarvalhoAndrade, Margareth SpanglerBahia, Maria TerezinhaMosqueira, Vanessa Carla Furtado2023-10-302023-10-302022SIQUEIRA, R. P. et al. In vitro interaction of polyethylene glycol‐block‐poly (D,L‐lactide) nanocapsule devices with host cardiomyoblasts and trypanosoma cruzi‐infective forms. Parasitology Research, v. 121, p. 2861–2874, 2022. Disponível em: <https://link.springer.com/article/10.1007/s00436-022-07618-0>. Acesso em: 01 ago. 2023.1432-1955http://www.repositorio.ufop.br/jspui/handle/123456789/17684Chagas disease, caused by the protozoan Trypanosoma cruzi, is an important public health problem in Latin America. Nanoencapsulation of anti-T. cruzi drugs has signifcantly improved their efcacy and reduced cardiotoxicity. Thus, we investigated the in vitro interaction of polyethylene glycol-block-poly(D,L-lactide) nanocapsules (PEG-PLA) with trypomas- tigotes and with intracellular amastigotes of the Y strain in cardiomyoblasts, which are the infective forms of T. cruzi, using fuorescence and confocal microscopy. Fluorescently labeled nanocapsules (NCs) were internalized by non-infected H9c2 cells toward the perinuclear region. The NCs did not induce signifcant cytotoxicity in the H9c2 cells, even at the highest concentrations and interacted equally with infected and non-infected cells. In infected cardiomyocytes, NCs were distrib- uted in the cytoplasm and located near intracellular amastigote forms. PEG-PLA NCs and trypomastigote form interactions also occurred. Altogether, this study contributes to the development of engineered polymeric nanocarriers as a platform to encapsulate drugs and to improve their uptake by diferent intra- and extracellular forms of T. cruzi, paving the way to fnd new therapeutic strategies to fght the causative agent of Chagas disease.en-USrestritoCell uptakeArtigo publicado em periodicohttps://link.springer.com/article/10.1007/s00436-022-07618-0https://doi.org/10.1007/s00436-022-07618-0