Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production.

Grenfell, Rafaella Fortini QueirozSilva, Eduardo de Almeida Marques daTestasicca, Miriam Conceição de SouzaCoelho, Eduardo Antônio FerrazFernandes, Ana Paula Salles MouraAfonso, Luís Carlos CroccoRezende, Simone Aparecida2014-11-192014-11-192010GRENFELL, R. F. Q. et al. Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production. Memórias do Instituto Oswaldo Cruz, v. 105, p. 818-822, 2010. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600015&lng=en&nrm=iso&tlng=en>. Acesso em: 28 ago. 2014.0074-0276http://www.repositorio.ufop.br/handle/123456789/3967This study evaluated two vaccine candidates for their effectiveness in protecting BALB/c mice against Leishma¬nia chagasi infection. These immunogenic preparations were composed of Leishmania amazonensis or Leishmania braziliensis antigenic extracts in association with saponin adjuvant. Mice were given three subcutaneous doses of one of these vaccine candidates weekly for three weeks and four weeks later challenged with promastigotes of L. chagasi by intravenous injection. We observed that both vaccine candidates induced a significant reduction in the parasite load of the liver, while the L. amazonensis antigenic extract also stimulated a reduction in spleen parasite load. This protection was associated with a suppression of both interleukin (IL)-10 and IL-4 cytokines by spleen cells in response to L. chagasi antigen. No change was detected in the production of IFN-γ. Our data show that these im-munogenic preparations reduce the type 2 immune response leading to the control of parasite replication.en-USVaccineLeishmania chagasiLeishmania braziliensisLeishmania amazonensisAntigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production.Artigo publicado em periodicoO periódico Memórias do Instituto Oswaldo Cruz permite que o Repositório Institucional da Universidade Federal de Ouro Preto (UFOP) deposite uma cópia eletrônica dos artigos publicados por esse periódico em que ao menos um dos autores faça parte da comunidade cientifica da UFOP. Fonte: Licença concedida mediante prenchimento de formulário enviado no dia 12 dez. 2013.http://dx.doi.org/10.1590/S0074-02762010000600015