Brito, Rory Cristiane Fortes deCardoso, Jamille Mirelle de OliveiraReis, Levi Eduardo SoaresMathias, Fernando Augusto SiqueiraSoares, Rodrigo Dian de Oliveira AguiarCarvalho, Andréa Teixeira deRoatt, Bruno MendesOliveira, Rodrigo Corrêa deRuiz, Jeronimo ConceiçãoResende, Daniela de MeloReis, Alexandre Barbosa2020-05-082020-05-082019BRITO, R. C. F. et al. Synthetic peptides elicit strong cellular immunity in Visceral Leishmaniasis natural reservoir and contribute to long-lasting polyfunctional T-cells in BALB/c mice. Vaccines, v. 7, n. 4, p. 162, out. 2019. Disponível em: <https://www.mdpi.com/2076-393X/7/4/162>. Acesso em: 10 fev. 2020.2076-393Xhttp://www.repositorio.ufop.br/handle/123456789/12172Reverse vaccinology or immunoinformatics is a computational methodology which integrates data from in silico epitope prediction, associated to other important information as, for example, the predicted subcellular location of the proteins used in the design of the context of vaccine development. This approach has the potential to search for new targets for vaccine development in the predicted proteome of pathogenic organisms. To date, there is no effective vaccine employed in vaccination campaigns against visceral leishmaniasis (VL). For the first time, herein, an in silico, in vitro, and in vivo peptide screening was performed, and immunogenic peptides were selected to constitute VL peptide-based vaccines. Firstly, the screening of in silico potential peptides using dogs naturally infected by L. infantum was conducted and the peptides with the best performance were selected. The mentioned peptides were used to compose Cockt-1 (cocktail 1) and Cockt-2 (cocktail 2) in combination with saponin as the adjuvant. Therefore, tests for immunogenicity, polyfunctional T-cells, and the ability to induce central and effector memory in T-lymphocytes capacity in reducing the parasite load on the spleen for Cockt-1 and Cockt-2 were performed. Among the vaccines under study, Cockt-1 showed the best results, eliciting CD4+ and CD8+ polyfunctional T-cells, with a reduction in spleen parasitism that correlates to the generation of T CD4+ central memory and T CD8+ effector memory cells. In this way, our findings corroborate the use of immunoinformatics as a tool for the development of future vaccines against VL.en-USabertoReverse vaccinologyImmunoinformaticsPeptide-based vaccineLeishmania infantumRational design of vaccinesArtigo publicado em periodicoThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Fonte: o próprio artigo.https://doi.org/10.3390/vaccines7040162