Chaves, Jaísa OliveiraFernandes, Angelica Maria de FreitasParreiras, Paola MachadoPassos, Maria CristinaCunha, Luciana Rodrigues daSalierno, Camila Carvalho Menezes2023-10-042023-10-042022CHAVES, J. O. et al. Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality. Revista Brasileira de Saúde Materno Infantil, v. 22, n. 4, p. 863-870, out./dez. 2022. Disponível em: <https://www.scielo.br/j/rbsmi/a/STXXz4nD3JNhhjYvHNR84rk/?format=pdf&lang=en>. Acesso em: 01 ago. 2023.1806-9304http://www.repositorio.ufop.br/jspui/handle/123456789/17518Objectives: microbiological quality of raw human milk (HM) from donors at the HMB (Human Milk Bank) of Santa Casa de Misericórdia de Ouro Preto was evaluated. Methods: the microbiological quality of milk from each of 10 donors in triplicate (30 samples) was evaluated by enumeration of Staphylococcus aureus and total aerobic mesophiles (surface plating) and total and thermotolerant coliforms (Most Probable Number - MPN). A questionnaire and a checklist were applied after the end of sample collection to verify the compliance of milk extraction and storage procedures by the donors. Results: inadequacies in the procedures for expression and storage of donated HM were observed. The presence of all microorganisms studied were verified in the milk of all donors, and in some cases exceeded the safety limits. The checklist indicated that some necessary hygienic-sanitary procedures were not carefully performed, making it important to properly guide the donors. Conclusions: this study points to an unfavorable scenario for the safety and quality of donated HM, and it is important to encourage further studies to be conducted so that the Brazilian network of HMB adopts stricter criteria for instructions on procedures and support for HM donors.en-USabertoDonorFood microbiologyHuman milkMilk banksQuality controlArtigo publicado em periodicoThis article is published in Open Access under the Creative Commons Attribution license, which allows use, distribution, and reproduction in any medium, without restrictions, as long as the original work is correctly cited. Fonte: PDF do artigo.https://doi.org/10.1590/1806-9304202200040008