Pedro, Guilherme ArantesAmaral, Mateus de SouzaPereira, Félix MonteiroFlumignan, Danilo LuizRós, Patrícia Caroline Molgero daReis, Cristiano Eduardo RodriguesSilva, Messias Borges2023-05-232023-05-232022PEDRO, G. A. et al. Highly wet chlorella minutissima biomass for in situ biodiesel production and residual biomass rich in labile carbohydrates. BioEnergy Research, v. 15, p. 154–165, 2022. Disponível em: <https://link.springer.com/article/10.1007/s12155-021-10295-z>. Acesso em: 11 out. 2022.1939-1234http://www.repositorio.ufop.br/jspui/handle/123456789/16651This study evaluated the use of biomass of Chlorella minutissima grown in 40-L photobioreactors using a Guillard f/2 biomass at over 80% moisture content as feedstock to produce ethyl esters. The study design investigated the infuence of acid catalyst type (HCl or H2SO4) and concentration (0.3 or 0.6 mol L−1), co-solvent to ethanol ratio (1:10 or 1:5 v/v), and acyl acceptor to biomass ratio (25 mL g−1 or 50 mL g−1) on ester yield and conversion. An L8 Taguchi design was used to evaluate the infuence of the factors, demonstrating a range of conversion ranging from 37 to 98%. Furthermore, the efects of reaction conditions on the residual biomass were assessed by measuring the concentration of acid-labile carbohydrates, which could be utilized in integrated sugar- and lipid-based algal refneries. It was found that in situ transesterifcation using HCl can provide easily accessible and fermentable carbohydrates at concentrations of about 32 wt% in residual biomass. In this sense, the fndings of this study demonstrate a further step in the integration of microalgae biomass in the development of liquid-fuel biorefneries.en-USrestritoFatty acid ethyl esterMicroalgaeArtigo publicado em periodicohttps://link.springer.com/article/10.1007/s12155-021-10295-zhttps://doi.org/10.1007/s12155-021-10295-z