Machado, Christina Monerat ToledoÁvila, Ricardo Andrez Machado deNGuyen, ChristopheGranier, ClaudeBueno, Lilian LacerdaCarneiro, Cláudia MartinsSouza, Daniel MenezesCarneiro, Rubens AntônioChávez Orlórtegui, CarlosFujiwara, Toshio2016-01-282016-01-282015MACHADO, C. M. T. et al. Immunodiagnosis of canine visceral leishmaniasis using mimotope peptides selected from phage is played combinatorial libraries. BioMed Research International, v. 2015, p. 1-10, 2015. Disponível em: <http://www.hindawi.com/journals/bmri/2015/401509/>. Acesso em: 15 out. 2015.2314-6141http://www.repositorio.ufop.br/handle/123456789/6274ELISA and RIFI are currently used for serodiagnosis of canine visceral leishmaniasis (CVL). The accuracy of these tests is controversial in endemic areas where canine infections by Trypanosoma cruzi may occur.We evaluated the usefulness of synthetic peptides that were selected through phage display technique in the serodiagnosis of CVL. Peptides were chosen based on their ability to bind to IgGs purified from infected dogs pooled sera. We selected three phage clones that reacted only with those IgGs. Peptideswere synthesized, polymerizedwith glutaraldehyde, and used as antigens in ELISA assays. Each individual peptide or a mix of them was reactive with infected dogs serum. The assay was highly sensitive and specific when compared to soluble Leishmania antigen that showed cross-reactivity with anti-T. cruzi IgGs. Our results demonstrate that phage display technique is useful for selection of peptides that may represent valuable synthetic antigens for an improved serodiagnosis of CVLen-USArtigo publicado em periodicoThis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: o próprio artigo.http://dx.doi.org/10.1155/2015/401509