Silva, Regislainy Gomes daAlmeida, Tamires CunhaReis, Adriana Cotta CardosoVieira Filho, Sidney AugustoBrandão, Geraldo CélioSilva, Glenda Nicioli daSousa, Hildeberto Caldas deAlmeida, Vera Lúcia deLopes, Júlio César DiasSouza, Gustavo Henrique Bianco de2021-09-162021-09-162020SILVA, R. G. da. et al. In silico pharmacological prediction and cytotoxicity of flavonoids glycosides identified by UPLC-DAD-ESI-MS/MS in extracts of Humulus lupulus leaves cultivated in Brazil. Natural Product Research, ago. 2020. Disponível em: <https://www.tandfonline.com/doi/abs/10.1080/14786419.2020.1803308>. Acesso em: 12 maio 2021.1478-6427http://www.repositorio.ufop.br/jspui/handle/123456789/13744Ethanolic (EB) extract and hexanic (SH) and hydromethanolic (SEM) sub-extracts of Humulus lupulus leaves were submitted to cytotoxicity evaluation and to phytochemical methods. The effect of EB and SEM on cellular cycle was evaluated by propidium iodide method and the phases were quantified through flow cytometry. The cytotoxicity assessment was done using T24 and MRC5 cells, with EB and SEM (25–1200 mg/mL). By means of UPLC-DADMS/MS data were identified the flavonoids astragaline, nicotiflorin, kaempferol-7-O-rutinoside, robinin, hyperin, rutin, quercetin-7-Orutinoside and manghaslin. EB (800 mg/mL) and SEM (1200 mg/mL) reduces the T24 cell viability. These extracts at 25 mg/mL stimulate the growth of MRC5 cells, evidencing a selective cytotoxicity. After 24 h of the treatment with extracts was not observed cycle arrest of T24 cells. The bioactivity prediction of the flavonoids was evaluated in silico through in house Active-IT software and PASSonline which indicated potential activity as antitumoral, cytotoxic, anti-inflammatory, antiparasitic, antimicrobial, antiviral and others.en-USrestritoArtigo publicado em periodicohttps://www.tandfonline.com/doi/abs/10.1080/14786419.2020.1803308https://doi.org/10.1080/14786419.2020.1803308