Antigenic peptides capable of inducing specific antibodies for detection of the major alterations found in type 2B von willebrand disease.

Paro, Marina de OliveiraFerreira, Cyntia SilvaVieira, Fernanda SilvaSantana, Marcos Aurélio deBorges, William de CastroLopes, Maria Sueli Silva NamenLeclercq, Sophie YvetteRodrigues, Cibele VellosoAndrade, Milton Hércules Guerra de2015-03-122015-03-122013PARO, M. de O. et al. Antigenic peptides capable of inducing specific antibodies for detection of the major alterations found in type 2B von willebrand disease. International Journal of Peptides, v. 2013, p. 1-6, 2013. Disponível em: <http://www.hindawi.com/journals/ijpep/2013/590329/>. Acesso em: 08 nov. 2014.1573-3904http://www.repositorio.ufop.br/handle/123456789/4620VonWillebrand disease (VWD) is an inherited hemorrhagic disorder promoted by either quantitative or qualitative defects of the von Willebrand factor (VWF). The disease represents the most common human coagulopathy afflicting 1.3% of the population. Qualitative defects are subdivided into four subtypes and classified according to the molecular dysfunction of the VWF. The differential diagnosis of the VWD is a difficult task, relying on a panel of tests aimed to assess the plasma levels and function of the VWF. Here, we propose biochemical approaches for the identification of structural variants of the VWF. A bioinformatic analysis was conducted to design seven peptides amongwhich threewere representatives of specific amino acid sequences belonging to normal VWF and four encompassed sequences found in the most common VWD subtype 2B. These peptides were used to immunize mice, after which, peptide-specific immunoglobulins were purified. This resulted in four Ig preparations capable of detecting alterations in the subtype 2B VWD plus additional three antibody fractions targeting the normal VWF. The panel of antibodies could serve many applications among them (1) assessment of VWF: antigen interaction, (2) VWF multimer analysis, and (3) production of monoclonal antibodies against VWF for therapeutic purposes as in thrombotic thrombocytopenic purpura.en-USAntigenic peptides capable of inducing specific antibodies for detection of the major alterations found in type 2B von willebrand disease.Artigo publicado em periodicoThis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: International Journal of Peptides <http://www.hindawi.com/journals/ijpep/> . Acesso em: 27 nov. 2014.http://dx.doi.org/10.1155/2013/590329