Moraes, Sabrina Zelice da Cruz deShan, Andrea Yu Kwan VillarMelo, Marlange Almeida OliveiraSilva, Juliane Pereira daPassos, Fabiolla Rocha SantosGraça, Ariel de SouzaAraújo, Brancilene Santos deQuintans, Jullyana de Souza SiqueiraQuintans Júnior, Lucindo JoséBarreto, Emiliano de OliveiraBrandão, Geraldo CélioEstevam, Charles dos Santos2021-12-062021-12-062020MORAES, S. Z. da C. de et al. Antinociceptive and anti-inflammatory effect of Poincianella pyramidalis (Tul.) L.P. Queiroz. Journal of Ethnopharmacology, p. 112563, 2020. Disponível em: <https://www.sciencedirect.com/science/article/abs/pii/S0378874119319257?via%3Dihub>. Acesso em: 10 jun. 2021.0378-8741http://www.repositorio.ufop.br/jspui/handle/123456789/14096Ethnopharmacology relevance: Poncianella pyramidalis (Leguminosae) is a Caatinga plant used in folk medicine because of its pharmacological properties, which include anti-inflammatory action. However, chemical compounds re sponsible for this effect have not yet been identified. Aim of the study: This study aimed to evaluate the antioxidant, antinociceptive and anti-inflammatory effects of the ethyl acetate fraction from the inner bark of P. pyramidalis. Material and methods: Total phenol content (TP) was estimated using the Folin-Ciocalteu reagent, while in vitro antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Chemical identification was done using LC-PDA/MS and LC-ESI/MS/MS. In vivo antinociceptive and anti-in flammatory properties were investigated using formalin, mechanical hypernociception and carrageenan-induced pleurisy assays in mice. Results: TP was 525.08 ± 17.49 μg mg−1 gallic acid equivalent. The ethyl acetate fraction (EAF) inhibited 87.76% of the DPPH radical with an EC50 of 22.94 μg mL−1 and Antioxidant Activity Index of 1.74. LC-PDA/MS and LC-ESI/MS/MS identified 15 compounds that are mostly derived from gallic and ellagic acids. Regarding in vivo antinociceptive and anti-inflammatory activity, EAF (100 mg kg-1) significantly reduced the nociceptive response in the second phase of the formalin assay by 50% (p < 0.01) compared with the control group. In the hypernociception test, a significant (p < 0.001) anti-hyperalgesic effect of EAF (100 mg kg-1) was observed up to the third hour of evaluation (p < 0.001). In the carrageenan assay, EAF (100 mg kg-1) was shown to inhibit protein extravasation, increase total leukocytes and neutrophils, and inhibit mononuclear cells. Conclusion: These results demonstrate EAF from the inner bark of P. pyramidalis has strong in vitro antioxidant effect as well as in vivo antinociceptive and anti-inflammatory activities, which may be attributed to the bark being rich in phenolic compounds derived from gallic acid.en-USrestritoPhenolicCarrageenanHyperalgesicHypernociceptionArtigo publicado em periodicohttps://www.sciencedirect.com/science/article/abs/pii/S0378874119319257?via%3Dihubhttps://doi.org/10.1016/j.jep.2020.112563