Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae.

dc.contributor.authorPereira, Michele B. P.
dc.contributor.authorTisi, Renata
dc.contributor.authorFietto, Luciano Gomes
dc.contributor.authorCardoso, Anamaria de Souza
dc.contributor.authorFrança, Mônica M.
dc.contributor.authorCarvalho, Fernanda Machado de
dc.contributor.authorTrópia, Maria José Magalhães
dc.contributor.authorMartegani, Enzo
dc.contributor.authorCastro, Ieso de Miranda
dc.contributor.authorBrandão, Rogélio Lopes
dc.date.accessioned2012-07-18T18:04:35Z
dc.date.available2012-07-18T18:04:35Z
dc.date.issued2008
dc.description.abstractThe plasma membrane H1-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H1-ATPase shares some similarities with the sugar-induced activation of the enzyme. Phospholipase C and protein kinase C activities are essential for this activation process while Gpa2p, a G protein involved in the glucose-induced activation of the ATPase, is not required. CCCP also induces a phospholipase Cdependent increase in intracellular calcium. Moreover, we show that the availability of extracellular calcium is required for CCCP stimulation of H1-ATPase, suggesting a possible connection between calcium signaling and activation of ATPase.pt_BR
dc.identifier.citationPEREIRA, M. B. P. et al. Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae. FEMS Yeast Research, v. 8, n.4, p.622-630, jun. 2008. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1567-1364.2008.00380.x/pdf>. Acesso em: 18 jul. 2012.pt_BR
dc.identifier.issn15671364
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/1164
dc.language.isoen_USpt_BR
dc.rights.licenseO Periódico FEMS Yeast Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 500810576.
dc.subjectDepolarizing compoundspt_BR
dc.subjectSaccharomyces cerevisiaept_BR
dc.titleCarbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae.pt_BR
dc.typeArtigo publicado em periodicopt_BR

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