Antibodies from dogs with canine visceral leishmaniasis (CVL) recognise two proteins from the saliva of lutzomyia longipalpis.
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2007
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The saliva of the sand fly Lutzomyia longipalpis, a
major vector of Leishmania, exhibits pharmacological and
immunomodulatory activities that may facilitate entry and
establishment of parasites into the vertebrate host. Salivary
gland components of the sand fly are, therefore, potential
candidates in the development of a vaccine against human
leishmaniasis. With the objective of identifying sand fly saliva
proteins that could be used to immunise animals against
canine visceral leishmaniasis, we have evaluated anti-saliva
antibody reactivity using serum samples collected from dogs
naturally infected with Leishmania chagasi. Two proteins
with molecular weights of 28.6 and 47.3 kDa were recognised
by dog antibodies in Western blot assays. Protein bands were
excised from an SDS-PAGE gel and the sequences determined
by mass spectrometry. The proteins were identified as
LuLo-D7 and Lulo YELLOW, respectively. The significance
of these findings in the context of the development of multicomponent
vaccination experiments is discussed.
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BAHIA, D. et al. Antibodies from dogs with canine visceral leishmaniasis (CVL) recognise two proteins from the saliva of lutzomyia longipalpis. Parasitology Research, v. 100, p. 449-454, 2007. Disponível em: <http://link.springer.com/article/10.1007/s00436-006-0307-8>. Acesso em: 10 out. 2016.