Anti-inflammatory and antinociceptive activities of Campomanesia adamantium.
dc.contributor.author | Ferreira, Lidiane Cristina | |
dc.contributor.author | Guimarães, Andrea Grabe | |
dc.contributor.author | Paula, Carmem Aparecida de | |
dc.contributor.author | Michel, Marcela Carolina de Paula | |
dc.contributor.author | Guimarães, Raquel Gomes | |
dc.contributor.author | Rezende, Simone Aparecida | |
dc.contributor.author | Souza Filho, José Dias de | |
dc.contributor.author | Guimarães, Dênia Antunes Saúde | |
dc.date.accessioned | 2017-02-09T12:05:21Z | |
dc.date.available | 2017-02-09T12:05:21Z | |
dc.date.issued | 2013 | |
dc.description.abstract | Ethnopharmacological relevance: Campomanesia species are used in folk medicine as anti-inflammatory, anti-rheumatic, anti-diarrheal and hypocholesterolemic. Aim of the study: The present study investigated the in vivo anti-inflammatory and antinociceptive properties of ethyl acetate (AE) and aqueous (Aq) extracts from leaves of Campomanesia adamantium and in vitro anti-inflammatory activity of AE and its isolated flavonols, myricitrin and myricetin. Materials and methods: The antinociceptive activity of AE and Aq was evaluated using acetic acid- induced writhing and formalin methods. The in vivo anti-inflammatory effect of AE and Aq was evaluated using carrageenan-induced paw oedema in mice. AE, myricitrin and myricetin were evaluated for their abilities to modulate the production of NO, TNF-a and IL-10 in LPS/IFN-g stimulated J774.A1 macrophages. Results: It was found that orally administrated AE and Aq (125 and 250 mg/kg) inhibited carrageenan- induced paw oedema in mice. AE (125 and 250 mg/kg) and Aq (125 mg/kg) reduced the time to licking at the second phase of the formalin method in vivo in mice. AE (250 mg/kg) and Aq (125 mg/kg) also reduced the number of writhes. AE, myricitrin and myricetin inhibited NO (320 mg/mL and 6.25–100 mM, respectively) and TNF-a production by macrophages (320 mg/mL for AE, 100 mM for myricitrin and 25–100 mM for myricetin). AE (160 and 320 mg/mL), myricitrin (50 and 100 mM) and myricetin (25–100 mM) increased IL-10 production by macrophages. Conclusions: The ethyl acetate and aqueous extracts from Campomanesia adamantium showed anti- nociceptive and anti-inflammatory effects supporting the use of the plant in folk medicine. The results suggest that anti-oedematogenic effect promoted by aqueous extract involves several anti- inflammatory mechanisms of action. The antinociceptive effect shown by aqueous extract can be due to the modulation of release of inflammatory mediators involved in nociception. The anti-inflammatory effects of AE and of its isolated flavonols may be attributed to inhibition of pro-inflammatory cytokines production, TNF-a and NO and to the increased of IL-10 production. | pt_BR |
dc.identifier.citation | FERREIRA, L. C. et al. Anti-inflammatory and antinociceptive activities of Campomanesia adamantium. Journal of Ethnopharmacology, v. 145, p.100-108, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0378874112007349>. Acesso em: 10 out. 2016. | pt_BR |
dc.identifier.doi | https://doi.org/10.1016/j.jep.2012.10.037 | |
dc.identifier.issn | 0378-8741 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/7237 | |
dc.language.iso | en_US | pt_BR |
dc.rights | aberto | pt_BR |
dc.rights.license | O periódico Journal of Ethnopharmacology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 4243050204672. | |
dc.subject | Flavonols | pt_BR |
dc.title | Anti-inflammatory and antinociceptive activities of Campomanesia adamantium. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |