Performance of LBSap vaccine after intradermal challenge with L. infantum and saliva of Lu. longipalpis : immunogenicity and parasitological evaluation.

dc.contributor.authorRoatt, Bruno Mendes
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorSouza, Juliana Vitoriano de
dc.contributor.authorVital, Wendel Coura
dc.contributor.authorBraga, Samuel Leôncio
dc.contributor.authorOliveira, Rodrigo Corrêa de
dc.contributor.authorMartins Filho, Olindo Assis
dc.contributor.authorCarvalho, Andréa Teixeira de
dc.contributor.authorLana, Marta de
dc.contributor.authorGontijo, Nelder de Figueiredo
dc.contributor.authorMarques, Marcos José
dc.contributor.authorGiunchetti, Rodolfo Cordeiro
dc.contributor.authorReis, Alexandre Barbosa
dc.date.accessioned2015-01-22T14:54:23Z
dc.date.available2015-01-22T14:54:23Z
dc.date.issued2012
dc.description.abstractIn the last decade, the search for new vaccines against canine visceral leishmaniasis has intensified. However, the pattern related to immune protection during long periods after experimental infection in vaccine trials is still not fully understood. Herein, we investigated the immunogenicity and parasitological levels after intradermal challenge with Leishmania infantum plus salivary gland extract in dogs immunized with a vaccine composed of L. braziliensis antigens plus saponin as an adjuvant (LBSap vaccine). The LBSap vaccine elicited higher levels of total anti-Leishmania IgG as well as both IgG1 and IgG2. Furthermore, dogs vaccinated had increased levels of lymphocytes, particularly circulating B cells (CD21+) and both CD4+ and CD8+ T lymphocytes. LBSap also elicited an intense in vitro cell proliferation associated with higher levels of CD4+ T lymphocytes specific for vaccine soluble antigen and soluble lysate of L. infantum antigen even 885 days after experimental challenge. Furthermore, LBSap vaccinated dogs presented high IFN-c and low IL-10 and TGF-b1 expression in spleen with significant reduction of parasite load in this tissue. Overall, our results validate the potential of LBSap vaccine to protect against L. infantum experimental infection and strongly support further evaluation of efficiency of LBSap against CVL in natural infection conditions.pt_BR
dc.identifier.citationROATT, B. M. Performance of LBSap vaccine after intradermal challenge with L. infantum and saliva of Lu. longipalpis : immunogenicity and parasitological evaluation. Plos One, v. 7, n.11 p. e49780, 2012. Disponível em: <http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0049780>. Acesso em: 14 ago. 2014.pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0049780
dc.identifier.issn1932-6203
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4329
dc.language.isoen_USpt_BR
dc.rights.licenseOs trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <https://www.plos.org/open-access>. Acesso em: 03 jan. 2017.pt_BR
dc.subjectLeishmania infantumpt_BR
dc.titlePerformance of LBSap vaccine after intradermal challenge with L. infantum and saliva of Lu. longipalpis : immunogenicity and parasitological evaluation.pt_BR
dc.typeArtigo publicado em periodicopt_BR
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
ARTIGO_PerformanceLBSapVaccine.pdf
Tamanho:
887.08 KB
Formato:
Adobe Portable Document Format
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
2.57 KB
Formato:
Item-specific license agreed upon to submission
Descrição: