Glycerol utilization in Fusarium oxysporum var lini : regulation of transport and metabolism.
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1991
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Glycerol was transported in the fungus Fusarium oxysporum var. lini by a facilitated diffusion transport system
with a half-saturation constant, Ks, of 0.5 mM and a maximum velocity, Vmax, of 0.9 mmol (g dry wt)-l h-l at pH 5
and 25OC. 1,2-Propanediol was a competitive inhibitor of glycerol transport, but the cells did not actively
accumulate 1,2-propanediol. The transport system was partially constitutive. In cells grown in the presence of
glucose, glycerol was not transported, indicating that the synthesis of the system was under glucose repression.
Glycerol base and NADP+-dependent glycerol dehydrogenase activities were present under all physiological
conditions tested. A flavin-dependent glycerol phosphate dehydrogenase was induced only when glycerol was the
sole energy source in the medium. This enzyme, together with the transport system, constitute the regulated steps in
the glycerol metabolic pathway.
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CASTRO, I. de M.; DIAS, M. C. L. Glycerol utilization in Fusarium oxysporum var lini: regulation of transport and metabolism. Journal Of General Microbiology, v. 137, p.1497-1502, 1991. Disponível em: <http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-7-1497>. Acesso em: 10 jan. 2017.