A Leishmania hypothetical protein-containing liposome-based formulation is highly immunogenic and induces protection against visceral leishmaniasis.
dc.contributor.author | Ribeiro, Patrícia Aparecida Fernandes | |
dc.contributor.author | Dias, Daniel Silva | |
dc.contributor.author | Novais, Marcus V. M. | |
dc.contributor.author | Lage, Daniela Pagliara | |
dc.contributor.author | Tavares, Grasiele de Sousa Vieira | |
dc.contributor.author | Mendonça, Débora Vasconcelos Costa | |
dc.contributor.author | Oliveira, Jamil Silvano de | |
dc.contributor.author | Chávez Fumagalli, Miguel Angel | |
dc.contributor.author | Roatt, Bruno Mendes | |
dc.contributor.author | Duarte, Mariana Costa | |
dc.contributor.author | Souza, Daniel Menezes | |
dc.contributor.author | Ribeiro, Fernanda Ludolf | |
dc.contributor.author | Tavares, Carlos Alberto Pereira | |
dc.contributor.author | Oliveira, Mônica Cristina de | |
dc.contributor.author | Coelho, Eduardo Antônio Ferraz | |
dc.date.accessioned | 2019-04-02T18:20:34Z | |
dc.date.available | 2019-04-02T18:20:34Z | |
dc.date.issued | 2018 | |
dc.description.abstract | Leishmania proteins have been evaluated as vaccine candidates against leishmaniasis; however, most antigens present low immunogenicity and need to be added with immune adjuvants. A low number of licensed adjuvants exist on the market today; therefore, research conducted to produce new products is desirable. The present study sought to evaluate the immunogenicity and protective efficacy of a recombinant Leishmania hypothetical protein, namely LiHyR, administered with saponin or liposomes in BALB/c mice. Immunological and parasitological parameters were evaluated, and results showed significant protection against Leishmania infantum infection produced by both compositions in the immunized animals; however, this was not identified when the antigen was used alone. In addition, the liposomal formulation was more effective in inducing a polarized Th1 response in the vaccinated animals, which was maintained after challenge and reflected by lower parasitism found in all evaluated organs when the limiting dilution technique and RT-PCR assay were employed. The protected animals showed higher levels of protein and parasite-specific IFN-γ IL-2, IL-12, GM-CSF, and TNF-α, which were evaluated by capture ELISA and flow cytometry, in addition to a higher production of anti-protein and anti-parasite IgG2a antibodies, both before and after challenge. The Lip/rLiHyR combination induced higher IFN-γ production through both CD4+ and CD8+ T cell subtypes. Results indicate the possibility of using the LiHyR, containing a liposomal formulation, as a vaccine candidate against visceral leishmaniasis. | pt_BR |
dc.identifier.citation | RIBEIRO, P. A. F. et al. A Leishmania hypothetical protein-containing liposome-based formulation is highly immunogenic and induces protection against visceral leishmaniasis. Cytokine, v. 111, p. 131-139, nov. 2018. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1043466618303557?via%3Dihub>. Acesso em: 22 fev. 2019. | pt_BR |
dc.identifier.issn | 10434666 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/10910 | |
dc.identifier.uri2 | https://www.sciencedirect.com/science/article/pii/S1043466618303557 | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.subject | Adjuvants | pt_BR |
dc.subject | Vaccine | pt_BR |
dc.subject | Saponin | pt_BR |
dc.title | A Leishmania hypothetical protein-containing liposome-based formulation is highly immunogenic and induces protection against visceral leishmaniasis. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |
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