Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells : in vitro and in vivo traditional and replacement methods.
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2022
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Stevia urticifolia Thunb. is an underexploited herb possessing bioactive flavonoids, saponins, and
terpenoids. The aim of this study was to examine the antiproliferative and toxicogenetic properties
of the ethyl acetate extract from Stevia urticifolia aerial parts (EtAcSur) upon Artemia salina,
erythrocytes, Allium cepa and sarcoma 180 cells and fibroblasts, as well as in vivo studies on mice
to determine systemic, macroscopic, and behavioral alterations and bone marrow chromosomal
damage. The assessment using A. salina larvae and mouse blood cells revealed LC50 and EC50 values
of 68.9 and 113.6 µg/ml, respectively. Root growth and mitosis were inhibited by EtAcSur, and
chromosomal aberrations were detected only at 100 μg/ml. EtAcSur exhibited potent concentration-dependent viability reduction of S180 and L-929 cells and antioxidant capacity employing
ABTS• and DPPH•
. No previous in vivo studies were performed before with the EtAcSur. Signals of
acute toxicity were not observed at 300 mg/kg. Physiological and toxicological investigations at 25
and 50 mg/mg/day i.p. for 8 days did not markedly change body or organ relative weights, nor
patterns of spontaneous locomotor and exploratory activities. In contrast, clastogenic effects on
bone marrow were found at 50 mg/mg/day. EtAcSur was found to (1) produce toxicity in microcrustaceans, (2) capacity as free radical scavenger, (3) antimitotic, cytotoxic and clastogenic activties
upon vegetal and mammalian cells, and (4) lethality on both tumor and normal murine cells
indistinctly. In vivo damage systemic effects were not remarkable and clinical signals of toxicity
were not observed, suggesting the significant pharmacological potential of S. urticifolia for the
development of antineoplastic agents.
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Allium cepa, Chemoprevention, In vivo toxicity, Genotoxicity, Sarcoma 180
Citação
FERREIRA, P. M. P. et al. Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells: in vitro and in vivo traditional and replacement methods. Journal of Toxicology and Environmental Health-Part A-Current Issues, v. 85, p. 750-766, 2022. Disponível em: <https://www.tandfonline.com/doi/full/10.1080/15287394.2022.2081640>. Acesso em: 01 ago. 2023.