Identification of novel alleles conferring superior production of rose flavor phenylethyl acetate using polygenic analysis in yeast.

dc.contributor.authorCarvalho, Bruna Trindade de
dc.contributor.authorHolt, Sylvester
dc.contributor.authorSouffriau, Ben
dc.contributor.authorBrandão, Rogélio Lopes
dc.contributor.authorMoreno, Maria Remédios Foulquié
dc.contributor.authorTheveleina, Johan M.
dc.date.accessioned2018-05-14T14:51:38Z
dc.date.available2018-05-14T14:51:38Z
dc.date.issued2017
dc.description.abstractFlavor compound metabolism is one of the last areas in metabolism where multiple genes encoding biosynthetic enzymes are still unknown. A major challenge is the involvement of side activities of enzymes having their main function in other areas of metabolism. We have applied pooled-segregant whole-genome sequence analysis to identify novel Saccharomyces cerevisiae genes affecting production of phenylethyl acetate (2-PEAc). This is a desirable flavor compound of major importance in alcoholic beverages imparting rose- and honey-like aromas, with production of high 2-PEAc levels considered a superior trait. Four quantitative trait loci (QTLs) responsible for high 2-PEAc production were identified, with two loci each showing linkage to the genomes of the BTC.1D and ER18 parents. The first two loci were investigated further. The causative genes were identified by reciprocal allele swapping into both parents using clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9. The superior allele of the first major causative gene, FAS2, was dominant and contained two unique single nucleotide polymorphisms (SNPs) responsible for high 2-PEAc production that were not present in other sequenced yeast strains. FAS2 encodes the alpha subunit of the fatty acid synthetase complex. Surprisingly, the second causative gene was a mutant allele of TOR1, a gene involved in nitrogen regulation. Exchange of both superior alleles in the ER18 parent strain increased 2-PEAc production 70%, nearly to the same level as in the best superior segregant. Our results show that polygenic analysis combined with CRISPR/ Cas9-mediated allele exchange is a powerful tool for identification of genes encoding missing metabolic enzymes and for development of industrial yeast strains generating novel flavor profiles in alcoholic beverages.pt_BR
dc.identifier.citationCARVALHO, B. T. de et al. Identification of novel alleles conferring superior production of rose flavor phenylethyl acetate using polygenic analysis in yeast. mBio, v. 8, p. 1-21, 2017. Disponível em: <http://mbio.asm.org/content/8/6/e01173-17.full.pdf+html>. Acesso em: 05 abr. 2018.pt_BR
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/9932
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Fonte: o próprio artigo.pt_BR
dc.subjectFatty acid synthetasept_BR
dc.subjectRose flavorpt_BR
dc.subjectYeastpt_BR
dc.titleIdentification of novel alleles conferring superior production of rose flavor phenylethyl acetate using polygenic analysis in yeast.pt_BR
dc.typeArtigo publicado em periodicopt_BR

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