The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H -ATPase.
dc.contributor.author | Coccetti, Paola | |
dc.contributor.author | Tisi, Renata | |
dc.contributor.author | Martegani, Enzo | |
dc.contributor.author | Teixeira, Leonardo Souza | |
dc.contributor.author | Brandão, Rogélio Lopes | |
dc.contributor.author | Castro, Ieso de Miranda | |
dc.contributor.author | Thevelein, Johan Maria | |
dc.date.accessioned | 2012-07-19T14:25:50Z | |
dc.date.available | 2012-07-19T14:25:50Z | |
dc.date.issued | 1998 | |
dc.description.abstract | Addition of glucose to glucose-deprived cells of the yeast Saccharomyces cerevisiae triggers rapid turnover of phosphatidylinositol, phosphatidylinositol-phosphate and phosphatidylinositol 4,5-bisphosphate. Glucose stimulation of PI turnover was measured both as an increase in the specific ratio of 32P-labeling and as an increase in the level of diacylglycerol after addition of glucose. Glucose also causes rapid activation of plasma membrane H.-ATPase. We show that in a mutant lacking the PLC1 encoded phospholipase C, both processes were strongly reduced. Compound 48/80, a known inhibitor of mammalian phospholipase C, inhibits both processes. However, activation of the plasma membrane H.- ATPase is only inhibited by concentrations of compound 48/80 that strongly inhibit phospholipid turnover. Growth was inhibited by even lower concentrations. Our data suggest that in yeast cells, glucose triggers through activation of the PLC1 gene product a signaling pathway initiated by phosphatidylinositol turnover and involved in activation of the plasma membrane H.-ATPase. | pt_BR |
dc.identifier.citation | COCCETTI, P. et al. The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H -ATPase. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, v. 1405, n. 1-3, p. 147-154, out. 1998. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0167488998000998>. Acesso em: 19 jul. 2012. | pt_BR |
dc.identifier.issn | 01674889 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/1179 | |
dc.language.iso | en_US | pt_BR |
dc.rights.license | O periódico Biochimica et Biophysica Acta. Molecular Cell Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3266591238516. | |
dc.subject | Compound 48/80 | pt_BR |
dc.subject | Saccharomyces cerevisiae | pt_BR |
dc.subject | Glucose induction | pt_BR |
dc.subject | Signal transduction | pt_BR |
dc.subject | Medium acidification | pt_BR |
dc.title | The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H -ATPase. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |
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