In vitro interaction of polyethylene glycol‐block‐poly (D,L‐lactide) nanocapsule devices with host cardiomyoblasts and trypanosoma cruzi‐infective forms.

dc.contributor.authorSiqueira, Raoni Pais
dc.contributor.authorMilagre, Matheus Marques
dc.contributor.authorOliveira, Maria Alice de
dc.contributor.authorBranquinho, Renata Tupinambá
dc.contributor.authorTorchelsen, Fernanda Karoline Vieira da Silva
dc.contributor.authorLana, Marta de
dc.contributor.authorMachado, Marina Guimarães Carvalho
dc.contributor.authorAndrade, Margareth Spangler
dc.contributor.authorBahia, Maria Terezinha
dc.contributor.authorMosqueira, Vanessa Carla Furtado
dc.date.accessioned2023-10-30T18:42:22Z
dc.date.available2023-10-30T18:42:22Z
dc.date.issued2022pt_BR
dc.description.abstractChagas disease, caused by the protozoan Trypanosoma cruzi, is an important public health problem in Latin America. Nanoencapsulation of anti-T. cruzi drugs has signifcantly improved their efcacy and reduced cardiotoxicity. Thus, we investigated the in vitro interaction of polyethylene glycol-block-poly(D,L-lactide) nanocapsules (PEG-PLA) with trypomas- tigotes and with intracellular amastigotes of the Y strain in cardiomyoblasts, which are the infective forms of T. cruzi, using fuorescence and confocal microscopy. Fluorescently labeled nanocapsules (NCs) were internalized by non-infected H9c2 cells toward the perinuclear region. The NCs did not induce signifcant cytotoxicity in the H9c2 cells, even at the highest concentrations and interacted equally with infected and non-infected cells. In infected cardiomyocytes, NCs were distrib- uted in the cytoplasm and located near intracellular amastigote forms. PEG-PLA NCs and trypomastigote form interactions also occurred. Altogether, this study contributes to the development of engineered polymeric nanocarriers as a platform to encapsulate drugs and to improve their uptake by diferent intra- and extracellular forms of T. cruzi, paving the way to fnd new therapeutic strategies to fght the causative agent of Chagas disease.pt_BR
dc.identifier.citationSIQUEIRA, R. P. et al. In vitro interaction of polyethylene glycol‐block‐poly (D,L‐lactide) nanocapsule devices with host cardiomyoblasts and trypanosoma cruzi‐infective forms. Parasitology Research, v. 121, p. 2861–2874, 2022. Disponível em: <https://link.springer.com/article/10.1007/s00436-022-07618-0>. Acesso em: 01 ago. 2023.pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00436-022-07618-0pt_BR
dc.identifier.issn1432-1955
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/17684
dc.identifier.uri2https://link.springer.com/article/10.1007/s00436-022-07618-0pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectCell uptakept_BR
dc.titleIn vitro interaction of polyethylene glycol‐block‐poly (D,L‐lactide) nanocapsule devices with host cardiomyoblasts and trypanosoma cruzi‐infective forms.pt_BR
dc.typeArtigo publicado em periodicopt_BR

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura Disponível
Nome:
ARTIGO_VitroSInterationPolyethylene.pdf
Tamanho:
3.57 MB
Formato:
Adobe Portable Document Format
Descrição:

Licença do pacote

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura Disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: