Synthetic peptides elicit strong cellular immunity in Visceral Leishmaniasis natural reservoir and contribute to long-lasting polyfunctional T-cells in BALB/c mice.

dc.contributor.authorBrito, Rory Cristiane Fortes de
dc.contributor.authorCardoso, Jamille Mirelle de Oliveira
dc.contributor.authorReis, Levi Eduardo Soares
dc.contributor.authorMathias, Fernando Augusto Siqueira
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorCarvalho, Andréa Teixeira de
dc.contributor.authorRoatt, Bruno Mendes
dc.contributor.authorOliveira, Rodrigo Corrêa de
dc.contributor.authorRuiz, Jeronimo Conceição
dc.contributor.authorResende, Daniela de Melo
dc.contributor.authorReis, Alexandre Barbosa
dc.date.accessioned2020-05-08T17:46:42Z
dc.date.available2020-05-08T17:46:42Z
dc.date.issued2019
dc.description.abstractReverse vaccinology or immunoinformatics is a computational methodology which integrates data from in silico epitope prediction, associated to other important information as, for example, the predicted subcellular location of the proteins used in the design of the context of vaccine development. This approach has the potential to search for new targets for vaccine development in the predicted proteome of pathogenic organisms. To date, there is no effective vaccine employed in vaccination campaigns against visceral leishmaniasis (VL). For the first time, herein, an in silico, in vitro, and in vivo peptide screening was performed, and immunogenic peptides were selected to constitute VL peptide-based vaccines. Firstly, the screening of in silico potential peptides using dogs naturally infected by L. infantum was conducted and the peptides with the best performance were selected. The mentioned peptides were used to compose Cockt-1 (cocktail 1) and Cockt-2 (cocktail 2) in combination with saponin as the adjuvant. Therefore, tests for immunogenicity, polyfunctional T-cells, and the ability to induce central and effector memory in T-lymphocytes capacity in reducing the parasite load on the spleen for Cockt-1 and Cockt-2 were performed. Among the vaccines under study, Cockt-1 showed the best results, eliciting CD4+ and CD8+ polyfunctional T-cells, with a reduction in spleen parasitism that correlates to the generation of T CD4+ central memory and T CD8+ effector memory cells. In this way, our findings corroborate the use of immunoinformatics as a tool for the development of future vaccines against VL.pt_BR
dc.identifier.citationBRITO, R. C. F. et al. Synthetic peptides elicit strong cellular immunity in Visceral Leishmaniasis natural reservoir and contribute to long-lasting polyfunctional T-cells in BALB/c mice. Vaccines, v. 7, n. 4, p. 162, out. 2019. Disponível em: <https://www.mdpi.com/2076-393X/7/4/162>. Acesso em: 10 fev. 2020.pt_BR
dc.identifier.doihttps://doi.org/10.3390/vaccines7040162pt_BR
dc.identifier.issn2076-393X
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/12172
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Fonte: o próprio artigo.pt_BR
dc.subjectReverse vaccinologypt_BR
dc.subjectImmunoinformaticspt_BR
dc.subjectPeptide-based vaccinept_BR
dc.subjectLeishmania infantumpt_BR
dc.subjectRational design of vaccinespt_BR
dc.titleSynthetic peptides elicit strong cellular immunity in Visceral Leishmaniasis natural reservoir and contribute to long-lasting polyfunctional T-cells in BALB/c mice.pt_BR
dc.typeArtigo publicado em periodicopt_BR

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