DOCA-salt treatment enhances responses to endothelin-1 in murine corpus cavernosum.
dc.contributor.author | Carneiro, Fernando Silva | |
dc.contributor.author | Vitorino, Fernanda Regina Casagrande Giachini | |
dc.contributor.author | Lima, Victor Vitorino | |
dc.contributor.author | Carneiro, Zidonia N. | |
dc.contributor.author | Nunes, Kenia Pedrosa | |
dc.contributor.author | Ergul, Adviye | |
dc.contributor.author | Leite, Romulo | |
dc.contributor.author | Passaglia, Rita de Cassia Aleixo Tostes | |
dc.contributor.author | Webb, Robert Clinton | |
dc.date.accessioned | 2017-04-06T15:07:19Z | |
dc.date.available | 2017-04-06T15:07:19Z | |
dc.date.issued | 2008 | |
dc.description.abstract | The penis is kept in the flaccid state mainly via a tonic activity of norepinephrine and endothelins (ETs). ET-1 is important in salt-sensitive forms of hypertension. We hypothesized that cavernosal responses to ET-1 are enhanced in deoxycorticosterone acetate (DOCA)-salt mice and that blockade of ETA receptors prevents abnormal responses of the corpus cavernosum in DOCA-salt hypertension. Male C57BL/6 mice were unilaterally nephrectomized and treated for 5 weeks with both DOCA and water containing 1% NaCl and 0.2% KCl. Control mice were uninephrectomized and received tap water with no added salt. Animals received either the ETA antagonist atrasentan (5 mg·day−1·kg−1 body weight) or vehicle. DOCA-salt mice displayed increased systolic blood pressure (SBP), and treatment with atrasentan decreased SBP in DOCA-salt mice. Contractile responses in cavernosal strips from DOCA-salt mice were enhanced by ET-1, phenylephrine, and electrical field stimulation (EFS) of adrenergic nerves, whereas relaxations were not altered by IRL-1620 (an ETB agonist), acetylcholine, sodium nitroprusside, and EFS of nonadrenergic noncholinergic nerves. PD59089 (an ERK1/2 inhibitor), but not Y-27632 (a Rho-kinase inhibitor), abolished enhanced contractions to ET-1 in cavernosum from DOCA-salt mice. Treatment of DOCA-salt mice with atrasentan did not normalize cavernosal responses. In summary, DOCA-salt treatment in mice enhances cavernosal reactivity to contractile, but not to relaxant, stimuli, via ET-1/ETA receptor-independent mechanisms. | pt_BR |
dc.identifier.citation | CARNEIRO, F. S. et al. DOCA-salt treatment enhances responses to endothelin-1 in murine corpus cavernosum. Canadian Journal of Physiology and Pharmacology, v. 86, p. 320-328, 2008. Disponível em: <https://www.nrcresearchpress.com/doi/10.1139/Y08-031#.Xclvj1VKjm4>. Acesso em: 10 jan. 2017. | pt_BR |
dc.identifier.doi | https://dx.doi.org/10.1139%2FY08-031 | |
dc.identifier.issn | 1205-7541 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/7562 | |
dc.identifier.uri2 | https://www.nrcresearchpress.com/doi/10.1139/Y08-031#.Xclvj1VKjm4 | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.subject | Rho-kinase | pt_BR |
dc.subject | Atrasentan | pt_BR |
dc.title | DOCA-salt treatment enhances responses to endothelin-1 in murine corpus cavernosum. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |