Antiplasmodial activity and cytotoxicity, isolation of active alkaloids, and dereplication of Xylopia sericea leaves ethanol extract by UPLC-DAD-ESI-MS/MS.
dc.contributor.author | Gontijo, Douglas da Costa | |
dc.contributor.author | Brandão, Geraldo Célio | |
dc.contributor.author | Nascimento, Maria Fernanda Alves do | |
dc.contributor.author | Oliveira, Alaide Braga de | |
dc.date.accessioned | 2019-04-15T15:28:35Z | |
dc.date.available | 2019-04-15T15:28:35Z | |
dc.date.issued | 2019 | |
dc.description.abstract | Objectives: To assess the antiplasmodial activity of the ethanol extract of Xylopia sericea leaves, Annonaceae, often associated with antimalarial use and to perform a bioguided isolation of active compounds. Methods: Dereplication of ethanol extract by the UPLC-DAD-ESI-MS/MS technique allowed the identification of the major constituents, isolation and identification of alkaloids. The antiplasmodial and cytotoxic activity of the extract, fractions and isolated compounds was evaluated against the chloroquine-resistant W2 strain Plasmodium falciparum and HepG2 cells, respectively. Key findings: Ethanol extract showed high reduction of parasitemia as well as moderate cytotoxicity (86.5 3.0% growth inhibition at 50 lg/ml and CC50 72.1 5.1 lg/ml, respectively). A total of eight flavonoids were identified, and two aporphine alkaloids, anonaine and O-methylmoschatoline, were isolated. Anonaine disclosed significant antiplasmodial effect and moderate cytotoxicity (IC50 23.2 2.7 lg/ml, CC50 38.3 2.3 lg/ml, SI 1.6) while O-methylmoschatoline was not active against P. falciparum and showed a low cytotoxicity (33.5 1.9% growth inhibition at 50 lg/ml, CC50 274.4 0.5 lg/ml). Conclusions: Characterization of Xylopia sericea leaves ethanol extract by UPLCDAD-ESI-MS/MS as well as its antiplasmodial activity and the occurrence of anonaine and O-methylmoschatoline in this Xylopia species are reported by the first time. | pt_BR |
dc.identifier.citation | GONTIJO, D. da C. et al. Antiplasmodial activity and cytotoxicity, isolation of active alkaloids, and dereplication of Xylopia sericea leaves ethanol extract by UPLC-DAD-ESI-MS/MS. Journal of Pharmacy and Pharmacology, v. 71, n. 2, p. 260-269, fev. 2019. Disponível em: <https://onlinelibrary.wiley.com/doi/full/10.1111/jphp.13029>. Acesso em: 25 fev. 2019. | pt_BR |
dc.identifier.issn | 20427158 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/11021 | |
dc.identifier.uri2 | https://onlinelibrary.wiley.com/doi/full/10.1111/jphp.13029 | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.subject | Extract dereplication | pt_BR |
dc.subject | Flavonoids | pt_BR |
dc.title | Antiplasmodial activity and cytotoxicity, isolation of active alkaloids, and dereplication of Xylopia sericea leaves ethanol extract by UPLC-DAD-ESI-MS/MS. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |
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