In vitro activity of 2-pyridinecarboxylic acid against trypanosomes of the subgenus Schizotrypanum isolated from the bat Phyllostomus hastatus.

Resumo

The effect of 2-pyridinecarboxylic acid (picolinic acid) on trypanosomes of the subgenus Schizotrypanum isolated from the bat Phyllostomus hastatus was determined in this study. Picolinic acid, at 50 g mL-1, inhibited epimastigote growth by 99% after 12 days incubation. In addition, trypomastigote motility decreased by 50% after 6h and completely after 24h in the presence of 50 g mL-1 picolinic acid. The 50% cytotoxic concentration on HEp-2 cell line was 275 g mL-1 after 4 days incubation. Altogether, these results indicate higher toxicity against trypanosomes. The inhibitory effect of picolinic acid on epimastigote growth can be partially reversed by nicotinic acid and L tryptophan, suggesting a competitive inhibition. Furthermore, two anti-Trypanosoma (Schizotrypanum) cruzi drugs were also evaluated with regard to bat trypanosome growth. Benznidazole, at 50 g mL-1, inhibited epimastigote growth by 90% after 12 days incubation. Nifurtimox, at the same concentration, caused 96% growth inhibition after four days incubation. Corroborating a previous study, bat trypanosomes are a good model for screening new trypanocidal compounds. Moreover, they can be used to study many biological processes common to human pathogenic trypanosomatids.

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Bat trypanosome, Schizotrypanum, Antimicrobial activity

Citação

CORRÊA, P. R. et al. In vitro activity of 2-pyridinecarboxylic acid against trypanosomes of the subgenus Schizotrypanum isolated from the bat Phyllostomus hastatus. Acta Scientiarum. Biological Sciences, v. 33, n. 4, p. 437-443, 2011. Disponível em: <http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6482>. Acesso em: 20 ago. 2014.

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