Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.

dc.contributor.authorOldoni, Tatiane Luiza Cadorin
dc.contributor.authorMelo, Priscilla Siqueira
dc.contributor.authorMassarioli, Adna Prado
dc.contributor.authorMoreno, Ivani Aparecida Marchetto
dc.contributor.authorBezerra, Rosangela Maria Neves
dc.contributor.authorRosalen, Pedro Luiz
dc.contributor.authorSilva, Gil Valdo José da
dc.contributor.authorNascimento, Andréa Mendes do
dc.contributor.authorAlencar, Severino Matias de
dc.date.accessioned2016-07-29T16:51:53Z
dc.date.available2016-07-29T16:51:53Z
dc.date.issued2016
dc.description.abstractPurification and bioassay-guided fractionation were employed to isolate proanthocyanidins with antioxidant activity from peanut skin (Arachis hypogaea Runner 886). The crude extract was prepared with acetone (60% v/v) and purified using chromatographic methods, including a semipreparative HPLC technique. As a result, two proanthocyanidins were isolated and identified using NMR, epicatechin-(2 b?O?7, 4 b?8)-catechin (proanthocyanidin A1) and epicatechin-(b?2 O?7, 4 b?8)-epicatechin (proanthocyanidin A2). Despite the structural similarity, differences were observed in their antioxidant activity. Proanthocyanidin A1 proved to be more active, with EC50 value for DPPH radical scavenging of 18.25 lg/mL and reduction of Fe3+–TPTZ complex of 7.59 mmol/g, higher than that of synthetic antioxidant BHT. This compound evaluated by ABTS+_ was similar to that of natural quercetin. Therefore, peanut skin is an important source of bioactive compounds that may be used as a mild antioxidant for food preservation.pt_BR
dc.identifier.citationOLDONI,T. L. C. et al. Bioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques. Food Chemistry, v. 192, p. 306-312, 2016. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0308814615010092>. Acesso em: 16 jun. 2016.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.foodchem.2015.07.004
dc.identifier.issn0308-8146
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/6678
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseO periódico Food Chemistry concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3898810379758.pt_BR
dc.subjectPurificationpt_BR
dc.subjectChromatographypt_BR
dc.subjectAntioxidant activitypt_BR
dc.subjectPhenolic compoundspt_BR
dc.titleBioassay - guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.pt_BR
dc.typeArtigo publicado em periodicopt_BR

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