Effect of the trehalose levels on the screening of yeast as probiotic by in vivo and in vitro assays.
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Data
2008
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Resumo
Probiotics are viable defined microorganisms (bacteria or yeasts) that exert a beneficial effect on the health of
the host when ingested in adequate amounts. Screening for such biotherapeutic agents is commonly performed
by in vitro assays simulating gastrointestinal environment to determine the ability to survive in the digestive
tract. In the present study, the possibility of extrapolation of data obtained in in vitro assays to in vivo
conditions was studied using five Saccharomyces cerevisiae strains isolated from Brazilian Atlantic rain
forest. Trehalose contents and survival after exposure to a combination of physiological stresses generally
found in the gastrointestinal tract of humans were determined for the five yeasts and compared to the
behavior of Saccharomyces boulardii, a well-known probiotic. The results were completed with the
colonization capacity of the gastrointestinal tract of gnotobiotic mice by these yeast strains. Some results
obtained by in vitro assays are not confirmed by in vivo experiments, indicating that the extrapolation cannot
be always done.
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Saccharomyces boulardii, Saccharomyces cerevisiae, Stress
Citação
MARTINS, F. dos S. et al. Effect of the trehalose levels on the screening of yeast as probiotic by in vivo and in vitro assays. Brazilian Journal of Microbiology, v. 39, p. 50-55, 2008. Disponível em: <http://www.scielo.br/pdf/bjm/v39n1/a12v39n1.pdf>. Acesso em: 21 out. 2015