Laboratorial algorithm for serological diagnosis of visceral leishmaniasis using rK39-ICT, DAT-LPC and FC-Simplex IgG1.
dc.contributor.author | Vale, Isabela Natália Pascoal Campos do | |
dc.contributor.author | Saliba, Juliana Wilke | |
dc.contributor.author | Fonseca, Giuliana Schmidt França | |
dc.contributor.author | Pascoal, Vanessa Peruhype Magalhães | |
dc.contributor.author | Araújo, Fernanda Fortes de | |
dc.contributor.author | Xavier, Marcelo Antônio Pascoal | |
dc.contributor.author | Carvalho, Andréa Teixeira de | |
dc.contributor.author | Campos, Fernanda Magalhães Freire | |
dc.contributor.author | Andrade, Mariléia Chaves | |
dc.contributor.author | Lula, Jamille Fernandes | |
dc.contributor.author | Reis, Alexandre Barbosa | |
dc.contributor.author | Lemos, Elenice Moreira | |
dc.contributor.author | Carvalho, Sílvio Fernando Guimarães de | |
dc.contributor.author | Oliveira, Edward | |
dc.contributor.author | Martins Filho, Olindo Assis | |
dc.date.accessioned | 2021-09-03T15:49:34Z | |
dc.date.available | 2021-09-03T15:49:34Z | |
dc.date.issued | 2020 | pt_BR |
dc.description.abstract | The performance of distinct serological tests (rK39-ICT, IFAT, DAT-LPC, FC-Simplex IgG1) was assessed and a laboratorial algorithm was proposed for accurate diagnosis of VL. DAT-LPC and FC-Simplex IgG1 showed outstanding accuracy (AUC = 0.93) to identify VL patients. The use of a sequential serological algorithm (rK39-ICT screening followed by DAT-LPC or FC-Simplex IgG1) improved the global accuracy for VL (97.2%) diagnosis. An alternative approach for diagnosis of VL has been also assessed for interchangeable use of serum/whole blood lysate samples in DAT-LPC and FC-Simplex IgG1. Our data showed an outstanding agreement for the results obtained with whole blood lysate samples as compared to serum samples (DAT-LPC =100%; FC-Simplex IgG1 = 99%). Together, these findings provide insights to improve the current overall accuracy of VL diagnosis and present innovative laboratorial tests and alternative samples from use in public health services. | pt_BR |
dc.identifier.citation | VALE, I. N. P. C. do et al. Laboratorial algorithm for serological diagnosis of visceral leishmaniasis using rK39-ICT, DAT-LPC and FC-Simplex IgG1. Journal of Immunological Methods, v. 480, p. 112765, 2020. Disponível em: <https://www.sciencedirect.com/science/article/abs/pii/S002217592030034X?via%3Dihub>. Acesso em: 10 jun. 2021. | pt_BR |
dc.identifier.doi | https://doi.org/10.1016/j.jim.2020.112765 | pt_BR |
dc.identifier.issn | 0022-1759 | |
dc.identifier.uri | http://www.repositorio.ufop.br/jspui/handle/123456789/13646 | |
dc.identifier.uri2 | https://www.sciencedirect.com/science/article/abs/pii/S002217592030034X?via%3Dihub | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.title | Laboratorial algorithm for serological diagnosis of visceral leishmaniasis using rK39-ICT, DAT-LPC and FC-Simplex IgG1. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |
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