A specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis.

dc.contributor.authorOstolin, Thais Lopes Valentim Di Paschoale
dc.contributor.authorGusmão, Miriã Rodrigues
dc.contributor.authorMathias, Fernando Augusto Siqueira
dc.contributor.authorCardoso, Jamille Mirelle de Oliveira
dc.contributor.authorRoatt, Bruno Mendes
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorRuiz, Jeronimo Conceição
dc.contributor.authorResende, Daniela de Melo
dc.contributor.authorBrito, Rory Cristiane Fortes de
dc.contributor.authorReis, Alexandre Barbosa
dc.date.accessioned2023-10-06T20:52:10Z
dc.date.available2023-10-06T20:52:10Z
dc.date.issued2022pt_BR
dc.description.abstractThe development of an immunogenic, effective, and safe vaccine is essential as an alternative for disease control. The present study aimed to evaluate the immunogenicity and efficacy potential of a polyepitope T-cell antigen candidate against visceral leishmaniasis in a murine model. BALB/c mice were immunized with three doses subcutaneously with Poly-T Leish alone or adjuvanted with Saponin plus Monophosphoryl lipid A, with 15-day intervals between doses, and challenged with 107 stationary-phase Leishmania infantum promastigotes via tail vein. Immunogenicity and parasitism in spleen and liver of immunized mice were evaluated 45 days postchallenge. Our results revealed that the immunization with Poly-T Leish and Poly-T Leish/SM increases the percentage of specific T (CD4+ and CD8+) lymphocytes proliferation in vitro after antigen-specific stimulation. Also, Poly-T Leish and Poly-T Leish/SM groups showed a high percentage of IFN-γ and TNF-α-producing T cells, meanwhile, the Poly-T Leish/SM group also showed an increased percentage of multifunctional T cells producing double and triple-positive (IFN-γ+TNF-α+IL-2+) cytokines. The immunization with Poly-T Leish or Poly-T Leish/ SM stimulated a decreased IL-4 and IL-10 compared to the Saline and adjuvant group. Poly-T Leish/SM immunized mice exhibit a noteworthy reduction in the parasite burden (spleen and liver) through real-time PCR (96%). Moreover, we observed higher nitrite secretion in 120-hour stimulated-culture supernatant using Griess method. We demonstrated that the Poly-T Leish/SM candidate was potentially immunogenic, providing enhancement of protective immune mechanisms, and conferred protection reducing parasitism. Our candidate was considered potential against visceral leishmaniasis, and eventually, could be tested in phase I and II clinical trials in dogs.pt_BR
dc.identifier.citationOSTOLIN, T. L. V. D. P. et al. A specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis. Cellular Immunology, v. 380, artigo 104592, out. 2022. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0008874922001174?via%3Dihub>. Acesso em: 01 ago. 2023.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.cellimm.2022.104592pt_BR
dc.identifier.issn0008-8749
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/17535
dc.identifier.uri2https://www.sciencedirect.com/science/article/pii/S0008874922001174?via%3Dihubpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectVisceral leishmaniasispt_BR
dc.subjectLeishmania infantumpt_BR
dc.subjectProtozoan proteinspt_BR
dc.subjectPolyepitope vaccinept_BR
dc.subjectImmunogenicitypt_BR
dc.titleA specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis.pt_BR
dc.typeArtigo publicado em periodicopt_BR

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura Disponível
Nome:
ARTIGO_SpecificLeishmaniaInfantum.pdf
Tamanho:
2.37 MB
Formato:
Adobe Portable Document Format
Descrição:

Licença do pacote

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura Disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: